دورية أكاديمية
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody
| العنوان: | Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
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| المؤلفون: | Chun-Li Song, Qian Li, Yun-Peng Yu, Guan Wang, Jin-Peng Wang, Yang Lu, Ji-Chang Zhang, Hong-Ying Diao, Jian-Gen Liu, Yi-Hang Liu, Jia Liu, Ying Li, Dan Cai, Bin Liu |
| المصدر: | Brazilian Journal of Cardiovascular Surgery, Vol 30, Iss 2, Pp 159-163 (2015) |
| بيانات النشر: | Sociedade Brasileira de Cirurgia Cardiovascular, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Surgery LCC:Diseases of the circulatory (Cardiovascular) system |
| مصطلحات موضوعية: | Doença Arterial Coronariana, Stents Farmacológicos, Reestenose Coronária, Fator de Crescimento Endotelial Vascular, Antígenos CD 34, Surgery, RD1-811, Diseases of the circulatory (Cardiovascular) system, RC666-701 |
| الوصف: | AbstractIntroduction:Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel.Methods:Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody.Results:The results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody.Conclusion:Our study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-9741 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200005&lng=en&tlng=en; https://doaj.org/toc/1678-9741 |
| DOI: | 10.5935/1678-9741.20150016 |
| URL الوصول: | https://doaj.org/article/999d419fde7e4c75b9ff2d5817e28f62 |
| رقم الأكسشن: | edsdoj.999d419fde7e4c75b9ff2d5817e28f62 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16789741 |
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| DOI: | 10.5935/1678-9741.20150016 |