دورية أكاديمية

Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias

التفاصيل البيبلوغرافية
العنوان: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias
المؤلفون: Maroun Karam, Morgan Thenoz, Valérie Capraro, Jean-Philippe Robin, Christiane Pinatel, Agnès Lançon, Perrine Galia, David Sibon, Xavier Thomas, Sophie Ducastelle-Lepretre, Franck Nicolini, Mohamed El-Hamri, Youcef Chelghoun, Eric Wattel, Franck Mortreux
المصدر: Neoplasia: An International Journal for Oncology Research, Vol 16, Iss 1, Pp 21-30 (2014)
بيانات النشر: Elsevier, 2014.
سنة النشر: 2014
المجموعة: LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: Although numerous factors have been found to modulate hTERT transcription, the mechanism of its repression in certain leukemias remains unknown. We show here that DEK represses hTERT transcription through its enrichment on the hTERT promoter in cells from chronic and acute myeloid leukemias, chronic lymphocytic leukemia, but not acute lymphocytic leukemias where hTERT is overexpressed. We isolated DEK from the hTERT promoter incubated with nuclear extracts derived from fresh acute myelogenous leukemia (AML) cells and from cells expressing Tax, an hTERT repressor encoded by the human T cell leukemia virus type 1. In addition to the recruitment of DEK, the displacement of two potent known hTERT transactivators from the hTERT promoter characterized both AML cells and Tax-expressing cells. Reporter and chromatin immunoprecipitation assays permitted to map the region that supports the repressive effect of DEK on hTERT transcription, which was proportionate to the level of DEK-promoter association but not with the level of DEK expression. Besides hTERT repression, this context of chromatin redistribution of DEK was found to govern about 40% of overall transcriptional modifications, including those of cancer-prone genes. In conclusion, DEK emerges as an hTERT repressor shared by various leukemia subtypes and seems involved in the deregulation of numerous genes associated with leukemogenesis.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1476-5586
1522-8002
Relation: http://www.sciencedirect.com/science/article/pii/S1476558614800043; https://doaj.org/toc/1476-5586; https://doaj.org/toc/1522-8002
DOI: 10.1593/neo.131658
URL الوصول: https://doaj.org/article/9a70383a39c5494e816703b18ee8b225
رقم الأكسشن: edsdoj.9a70383a39c5494e816703b18ee8b225
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14765586
15228002
DOI:10.1593/neo.131658