دورية أكاديمية
Effect of superoxide on the development and maintenance of mechanical allodynia in a rat model of chronic post-ischemia pain
| العنوان: | Effect of superoxide on the development and maintenance of mechanical allodynia in a rat model of chronic post-ischemia pain |
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| المؤلفون: | Chang Gyu Han, Jae Kyung Han, Ki Bum Park, Kyung Hwa Kwak, Sung Sik Park, Dong Gun Lim |
| المصدر: | Korean Journal of Anesthesiology, Vol 63, Iss 2, Pp 149-156 (2012) |
| بيانات النشر: | Korean Society of Anesthesiologists, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Anesthesiology |
| مصطلحات موضوعية: | chronic post-ischemia pain, complex regional pain syndrome, mechanical allodynia, neuropathic pain, reactive oxygen species, superoxide, Anesthesiology, RD78.3-87.3 |
| الوصف: | BackgroundReactive oxygen species and inflammatory responses contribute to the development of neuropathic pain. Superoxide serves to mediate cell signaling processes and tissue injury during inflammation. We examined the effects of superoxide on the development and maintenance of mechanical allodynia, as well as its contribution to central sensitization in a superoxide-rich animal model of neuropathic pain.MethodsChronic post-ischemia pain (CPIP) was induced via the left hindpaw ischemia for 3 h, followed by reperfusion. Superoxide dismutase (4,000 U/kg, i.p.) was administered either 5 min before ischemia (BI), 5 min before reperfusion (BR), or 3 days after reperfusion (3AR). Withdrawal thresholds of the four paws were measured to assess the mechanical allodynia and the effects of circulating xanthine oxidase (XO)-mediated superoxide production. In addition, we measured the levels of N-methyl D-aspartate receptor subunit 1 phosphorylation (p-NR1) in the ipsilateral and contralateral spinal cord (L4-6), by Western blotting, to examine the superoxide-mediated central sensitization. Superoxide production was assessed by allopurinol-sensitive, XO-mediated lipid peroxidation of the spinal cord and gastrocnemius muscles.ResultsWithdrawal thresholds of forepaws did not vary across the 7 days of testing. In the hindpaws, both ipsilateral and contralateral mechanical allodynia was most attenuated in the BR group, followed by the BI and 3AR groups. The degree of NR1 activation was in contrast to the changes in the withdrawal thresholds.ConclusionsThese data suggest that superoxide is involved in the development and maintenance of mechanical allodynia, particularly via central sensitization in the spinal cord. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2005-6419 2005-7563 |
| Relation: | http://ekja.org/upload/pdf/kjae-63-149.pdf; https://doaj.org/toc/2005-6419; https://doaj.org/toc/2005-7563 |
| DOI: | 10.4097/kjae.2012.63.2.149 |
| URL الوصول: | https://doaj.org/article/9adbe320e65c40e1b26942e90fb86709 |
| رقم الأكسشن: | edsdoj.9adbe320e65c40e1b26942e90fb86709 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20056419 20057563 |
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| DOI: | 10.4097/kjae.2012.63.2.149 |