دورية أكاديمية
Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress
| العنوان: | Genome-wide identification and expression analysis of DREB genes in alfalfa (Medicago sativa) in response to cold stress |
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| المؤلفون: | Song Sheng, Xinyu Guo, Changzheng Wu, Yucheng Xiang, Shuhui Duan, Weiqin Yang, Wenrui Li, Fengchun Cao, Laihua Liu |
| المصدر: | Plant Signaling & Behavior, Vol 17, Iss 1 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Plant ecology LCC:Biology (General) |
| مصطلحات موضوعية: | medicago sativa, dreb protein, transcription factor, phylogenetic tree, cold stress, expression profiling, Plant ecology, QK900-989, Biology (General), QH301-705.5 |
| الوصف: | Dehydration-responsive element-binding proteins (DREBs) belong to members of the AP2/ERF transcription factor superfamily, which has been reported to involve various abiotic-stress responses and tolerance in plants. However, research on the DREB-family is still limited in alfalfa (Medicago sativa L.), a forage legume cultivated worldwide. The recent genome-sequence release of the alfalfa cultivar “XinJiangDaYe” allowed us to identify 172 DREBs by a multi-step homolog search. The phylogenetic analysis indicated that such MsDREBs could be classified into 5 groups, namely A-1 (56 members), A-2 (39), A-3 (3), A-4 (61) and 13 (A-5 (13), thus adding substantial new members to the DREB-family in alfalfa. Furthermore, a comprehensive survey in silico of conserved motif, gene structure, molecular weight, and isoelectric point (pI) as well as gene expression was conducted. The resulting data showed that, for cold-stress response, 33 differentially expressed MsDREBs were identified with a threshold of Log2-fold > 1, and most of which were transcriptionally upregulated within 48 h during a cold treatment(s). Moreover, the expression profiling of MsDREBs from two ecotypes of alfalfa subspecies i.e. M. sativa ssp. falcata (F56, from a colder region of Central Asia) and M. sativa ssp. sativa (B47, from Near East) revealed that most of the cold-stress responsive MsDREBs exhibited a significantly lower expression in F56, leading to a proposal of the existence of a distinct mechanism(s) for cold tolerance regulated by DREB-related action, which would have been evolved in alfalfa with a genotypic specificity. Additionally, by examining the transcriptome of a freezing-tolerance species (M. sativa cv. Zhaodong), eight DREBs were found to be implicated in a long-term freezing-stress adaptation with a great potential. Taken together, the current genome-wide identification in alfalfa points to the importance of some MsDREBs in the cold-stress response, providing some promising molecular targets to be functionally characterized for the improvement of cold tolerance in crops including alfalfa. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1559-2316 1559-2324 15592324 |
| Relation: | https://doaj.org/toc/1559-2316; https://doaj.org/toc/1559-2324 |
| DOI: | 10.1080/15592324.2022.2081420 |
| URL الوصول: | https://doaj.org/article/9b477c6d4cf1455db1de38d7821e0d3f |
| رقم الأكسشن: | edsdoj.9b477c6d4cf1455db1de38d7821e0d3f |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 15592316 15592324 |
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| DOI: | 10.1080/15592324.2022.2081420 |