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Towards a Rapid-Turnaround Low-Depth Unbiased Metagenomics Sequencing Workflow on the Illumina Platforms

التفاصيل البيبلوغرافية
العنوان: Towards a Rapid-Turnaround Low-Depth Unbiased Metagenomics Sequencing Workflow on the Illumina Platforms
المؤلفون: Winston Lian Chye Koh, Si En Poh, Chun Kiat Lee, Tim Hon Man Chan, Gabriel Yan, Kiat Whye Kong, Lalita Lau, Wai Yip Thomas Lee, Clark Cheng, Shawn Hoon, Yiqi Seow
المصدر: Bioengineering, Vol 10, Iss 5, p 520 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Technology
LCC:Biology (General)
مصطلحات موضوعية: metagenomic sequencing, host depletion, DNA/RNA library preparation, liquid biopsy, infectious disease, Technology, Biology (General), QH301-705.5
الوصف: Unbiased metagenomic sequencing is conceptually well-suited for first-line diagnosis as all known and unknown infectious entities can be detected, but costs, turnaround time and human background reads in complex biofluids, such as plasma, hinder widespread deployment. Separate preparations of DNA and RNA also increases costs. In this study, we developed a rapid unbiased metagenomics next-generation sequencing (mNGS) workflow with a human background depletion method (HostEL) and a combined DNA/RNA library preparation kit (AmpRE) to address this issue. We enriched and detected bacterial and fungal standards spiked in plasma at physiological levels with low-depth sequencing (
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2306-5354
Relation: https://www.mdpi.com/2306-5354/10/5/520; https://doaj.org/toc/2306-5354
DOI: 10.3390/bioengineering10050520
URL الوصول: https://doaj.org/article/9ba1cc59fa104afd9824f00cf9a83fa1
رقم الأكسشن: edsdoj.9ba1cc59fa104afd9824f00cf9a83fa1
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:23065354
DOI:10.3390/bioengineering10050520