دورية أكاديمية
Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus
| العنوان: | Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus |
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| المؤلفون: | Yuling Ding, Chanipa Jiratchayamaethasakul, Junseong Kim, Eun-A Kim, Soo-Jin Heo, Seung-Hong Lee |
| المصدر: | Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 1, Pp 33-41 (2020) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Biology (General) |
| مصطلحات موضوعية: | red sea cucumber (stichopus japonicus), ultrasonic extract, antioxidant activity, anti-melanogenesis, cosmeceuticals, Arctic medicine. Tropical medicine, RC955-962, Biology (General), QH301-705.5 |
| الوصف: | Objective: To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber, Stichopus japonicus, collected from Jeju Island. Methods: Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes (HaCaT) to determine its radical scavenging activity and protective effects against 2,2’-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. Anti-melanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and mushroom tyrosinase. Following the induction by α-melanocyte-stimulating hormone, the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity, melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related proteins (TRP-1, and TRP-2) were examined. Results: The ultrasonic extract of red sea cucumber significantly scavenged 2,2-diphenyl-1-picrylhydrazyl and alkyl radicals [IC50: (0.924±0.035) and (0.327±0.006) mg/mL, respectively], as well as showed a protective effect against oxidative stress and attenuated generation of intracellular reactive oxygen species on AAPH- induced HaCaT cells, with no cytotoxicity (12.5-400 μg/mL). The ultrasonic extract of red sea cucumber also exhibited a tyrosinase inhibitory effect [IC50: (2.750±0.006) mg/mL]. On α-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells, the ultrasonic extract of red sea cucumber (25-200 μg/mL) significantly inhibited not only melanin synthesis and tyrosinase activity, but also protein expressions of microphthalmia-associated transcriptional factor, tyrosinase, TRP-1, and TRP-2. Conclusions: The ultrasonic extract of red sea cucumber shows antioxidant and anti-melanogenic potential and may be a natural candidate for anti-aging as well as a whitening agent in the cosmeceuticals industry. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2588-9222 2221-1691 |
| Relation: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=1;spage=33;epage=41;aulast=Ding; https://doaj.org/toc/2588-9222 |
| DOI: | 10.4103/2221-1691.273092 |
| URL الوصول: | https://doaj.org/article/9ba6cc03fb43404687b0643b48f7c724 |
| رقم الأكسشن: | edsdoj.9ba6cc03fb43404687b0643b48f7c724 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25889222 22211691 |
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| DOI: | 10.4103/2221-1691.273092 |