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Real-Time Fluorometric Isothermal LAMP Assay for Detection of Chlamydia pecorum in Rapidly Processed Ovine Abortion Samples: A Veterinary Practitioner’s Perspective

التفاصيل البيبلوغرافية
العنوان: Real-Time Fluorometric Isothermal LAMP Assay for Detection of Chlamydia pecorum in Rapidly Processed Ovine Abortion Samples: A Veterinary Practitioner’s Perspective
المؤلفون: Tom Clune, Susan Anstey, Vasilli Kasimov, Caroline Jacobson, Martina Jelocnik
المصدر: Pathogens, Vol 10, Iss 9, p 1157 (2021)
بيانات النشر: MDPI AG, 2021.
سنة النشر: 2021
المجموعة: LCC:Medicine
مصطلحات موضوعية: Chlamydia pecorum, loop-mediated isothermal amplification, sheep, point-of-care, abortion, Medicine
الوصف: Traditional methods of detecting Chlamydia pecorum in tissue samples such as polymerase chain reaction or cell culture are laborious and costly. We evaluated the use of a previously developed C. pecorum LAMP assay using minimally processed ovine samples. Cotyledon (n = 16), foetal liver (n = 22), foetal lung (n = 2), and vaginal (n = 6) swabs, in addition to cotyledon (n = 6) and foetal liver (n = 8) tissue samples, were rapidly processed and used for LAMP testing without DNA extraction. Overall, LAMP test results were highly congruent with the in-house reference qPCR, with 80.43% (37/46; 72.73% positive agreement (PA); 84.75% negative agreement (NA)) overall agreeance for swab samples, and 85.71% (12/14; 80% PA; 88.89% NA) overall agreeance for tissue samples. Out of the 11 total discrepant results, discrepancy was mainly observed in samples (n = 10) with less than 100 copies/µL C. pecorum DNA. While sensitivity could be improved, the simplicity, low cost, and accuracy of detection makes this test amenable for use at point-of-care for detecting C. pecorum in sheep.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 10091157
2076-0817
Relation: https://www.mdpi.com/2076-0817/10/9/1157; https://doaj.org/toc/2076-0817
DOI: 10.3390/pathogens10091157
URL الوصول: https://doaj.org/article/9baa8e229644402591c3e3f72d269cd1
رقم الأكسشن: edsdoj.9baa8e229644402591c3e3f72d269cd1
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:10091157
20760817
DOI:10.3390/pathogens10091157