The Hillsborough County Health Department (HCHD) provides care for one-third of persons living with HIV/AIDS in Hillsborough County, Florida, who relied on public assistance for medical treatment. This study describes the demographic profile and trends for these patients. Data were obtained retrospectively from the HCHD’s electronic database for 2,298 patients who enrolled for HIV/AIDS care between January 1, 2000 and December 31, 2006. The patient sample included 32.6% women, 48.7% African Americans, 19.0% Hispanics, and 30.9% Whites, with mean age 39.7 ± 9.6 years. The proportion of Hispanic enrollees increased annually (p