دورية أكاديمية
Single-molecule analysis of the entire perfringolysin O pore formation pathway
| العنوان: | Single-molecule analysis of the entire perfringolysin O pore formation pathway |
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| المؤلفون: | Conall McGuinness, James C Walsh, Charles Bayly-Jones, Michelle A Dunstone, Michelle P Christie, Craig J Morton, Michael W Parker, Till Böcking |
| المصدر: | eLife, Vol 11 (2022) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | cholesterol dependent cytolysin, membrane pore, assembly kinetics, single-molecule fluorescence, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of all steps in the assembly pathway, we have used single-molecule fluorescence imaging to follow the dynamics of PFO on dye-loaded liposomes that lead to opening of a pore and release of the encapsulated dye. Formation of a long-lived membrane-bound PFO dimer nucleates the growth of an irreversible oligomer. The growing oligomer can insert into the membrane and open a pore at stoichiometries ranging from tetramers to full rings (~35 mers), whereby the rate of insertion increases linearly with the number of subunits. Oligomers that insert before the ring is complete continue to grow by monomer addition post insertion. Overall, our observations suggest that PFO membrane insertion is kinetically controlled. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/74901; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.74901 |
| URL الوصول: | https://doaj.org/article/9c52c20469604be898a9fcf4065fd2d3 |
| رقم الأكسشن: | edsdoj.9c52c20469604be898a9fcf4065fd2d3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.74901 |