دورية أكاديمية
A systems approach using OSMAC, Log P and NMR fingerprinting: An approach to novelty
| العنوان: | A systems approach using OSMAC, Log P and NMR fingerprinting: An approach to novelty |
|---|---|
| المؤلفون: | Miaomiao Liu, Tanja Grkovic, Xueting Liu, Jianying Han, Lixin Zhang, Ronald J. Quinn |
| المصدر: | Synthetic and Systems Biotechnology, Vol 2, Iss 4, Pp 276-286 (2017) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Biotechnology LCC:Biology (General) |
| مصطلحات موضوعية: | NMR fingerprints, OSMAC, Metabolites, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5 |
| الوصف: | The growing number of sequenced microbial genomes has revealed a remarkably large number of secondary metabolite biosynthetic clusters for which the compounds are still unknown. The aim of the present work was to apply a strategy to detect newly induced natural products by cultivating microorganisms in different fermentation conditions. The metabolomic analysis of 4160 fractions generated from 13 actinomycetes under 32 different culture conditions was carried out by 1H NMR spectroscopy and multivariate analysis. The principal component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between those samples within PC1 and PC2. The fractions with induced metabolites that are only produced under specific growth conditions was identified by PCA analysis. This method allows an efficient differentiation within a large dataset with only one fractionation step. This work demonstrates the potential of NMR spectroscopy in combination with metabolomic data analysis for the screening of large sets of fractions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-805X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405805X17300546; https://doaj.org/toc/2405-805X |
| DOI: | 10.1016/j.synbio.2017.10.001 |
| URL الوصول: | https://doaj.org/article/9c5949830f714e0ebc0d12887c64f4e5 |
| رقم الأكسشن: | edsdoj.9c5949830f714e0ebc0d12887c64f4e5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2405805X |
|---|---|
| DOI: | 10.1016/j.synbio.2017.10.001 |