دورية أكاديمية
أعرض في EDS

Unveiling microRNA-like small RNAs implicated in the initial infection of Fusarium oxysporum f. sp. cubense through small RNA sequencing

التفاصيل البيبلوغرافية
العنوان: Unveiling microRNA-like small RNAs implicated in the initial infection of Fusarium oxysporum f. sp. cubense through small RNA sequencing
المؤلفون: Lifei Xie, Yuntian Bi, Chengcheng He, Junjian Situ, Meng Wang, Guanghui Kong, Pinggen Xi, Zide Jiang, Minhui Li
المصدر: Mycology, Pp 1-16 (2024)
بيانات النشر: Taylor & Francis Group, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
LCC:Microbiology
مصطلحات موضوعية: Small RNAs, banana Fusarium Wilt, infection process, pathogenicity, Biology (General), QH301-705.5, Microbiology, QR1-502
الوصف: ABSTRACTBanana Fusarium wilt (BFW), caused by Fusarium oxysporum f. sp. cubense (Foc), poses a major challenge to the worldwide banana industry. Fungal microRNA-like small RNAs (milRNAs) play crucial roles in regulating fungal growth, conidiation, development, and pathogenicity. However, the milRNAs and their functions in the pathogenesis of Foc remain poorly understood. In this study, we employed high-throughput sequencing and bioinformatics to profile Foc sRNAs during both pure culture and early infection stages. Our analysis identified six milRNAs exhibiting significantly upregulated expression at the initial Foc infection. Of these, milR106’s biogenesis was found to be Dicer-dependent, whereas milR87, milR133, milR138, and milR148 were associated with Dicer and Argonaute proteins. Genetic manipulation and phenotype analysis confirmed that milR106 is crucial for Foc virulence by regulating conidiation, hydrogen peroxide sensitivity, and infective growth. Gene Ontology analysis of milRNA targets in the banana genome revealed enrichment in defence response to fungus and cellular response to hypoxia, implying the importance of these target genes in response to pathogen infection. In conclusion, our sRNA profiling of Foc identified several infection-induced milRNAs. The corresponding results provide valuable molecular targets for the development of an efficient strategy to control BFW.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 21501203
2150-1211
2150-1203
Relation: https://doaj.org/toc/2150-1203; https://doaj.org/toc/2150-1211
DOI: 10.1080/21501203.2024.2345917
URL الوصول: https://doaj.org/article/9d14f2d558f746d79cf04902ddc5900c
رقم الأكسشن: edsdoj.9d14f2d558f746d79cf04902ddc5900c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:21501203
21501211
DOI:10.1080/21501203.2024.2345917