دورية أكاديمية
Genome-Wide Identification and Characterization of Effector Candidates with Conserved Motif in Falciphora oryzae
| العنوان: | Genome-Wide Identification and Characterization of Effector Candidates with Conserved Motif in Falciphora oryzae |
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| المؤلفون: | Mengdi Dai, Zhenzhu Su, Xueming Zhu, Lin Li, Ziran Ye, Xiangfeng Tan, Dedong Kong, Xiaohong Liu, Fucheng Lin |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 1, p 650 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | genome-wide, effector, conserved motif, endophyte, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Microbes employ effectors to disrupt immune responses and promote host colonization. Conserved motifs including RXLR, LFLAK-HVLVxxP (CRN), Y/F/WxC, CFEM, LysM, Chitin-bind, DPBB_1 (PNPi), and Cutinase have been discovered to play crucial roles in the functioning of effectors in filamentous fungi. Nevertheless, little is known about effectors with conserved motifs in endophytes. This research aims to discover the effector genes with conserved motifs in the genome of rice endophyte Falciphora oryzae. SignalP identified a total of 622 secreted proteins, out of which 227 were predicted as effector candidates by EffectorP. By utilizing HMM features, we discovered a total of 169 effector candidates with conserved motifs and three novel motifs. Effector candidates containing LysM, CFEM, DPBB_1, Cutinase, and Chitin_bind domains were conserved across species. In the transient expression assay, it was observed that one CFEM and one LysM activated cell death in tobacco leaves. Moreover, two CFEM and one Chitin_bind inhibited cell death induced by Bax protein. At various points during the infection, the genes’ expression levels were increased. These results will help to identify functional effector proteins involving omics methods using new bioinformatics tools, thus providing a basis for the study of symbiosis mechanisms. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/1/650; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25010650 |
| URL الوصول: | https://doaj.org/article/a9d46475c8dd4c6e8d61f2a49283a330 |
| رقم الأكسشن: | edsdoj.9d46475c8dd4c6e8d61f2a49283a330 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25010650 |