دورية أكاديمية
Study the inhibitory effect and mechanism of the ethanol extract of deziyangxin on LLC cells
| العنوان: | Study the inhibitory effect and mechanism of the ethanol extract of deziyangxin on LLC cells |
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| المؤلفون: | Yi Yun, Jiang Yahui, Bai Bobo, Zhang Caifeng, Zhao Yanli |
| المصدر: | Heliyon, Vol 9, Iss 8, Pp e18712- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Chinese Tibetan medicine, Lewis lung cancer, Proliferation, Migration, PI3K/AKT, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Background: Chinese Tibetan medicine plays a crucial role in complementary anti-tumor treatments. This article aims to investigate the inhibitory effect of the alcoholic extract of Tibetan medicine Deziyangxin (DZYX) on the proliferation and migration of non-small cell lung cancer (NSCLC) cells, specifically LLC cells, as well as explore its potential mechanism of action. Methods: The effect of the alcoholic extract on LLC cell viability was assessed using the CCK-8 method. The proliferation of LLC cells was evaluated using the EdU (5-Acetyl-2'-deoxyuridine) assay. Transwell assays were conducted to measure cell metastasis. Western blot analysis was performed to assess the expression of Cleaved Caspase-3, Bcl-2, Beciln-1, indicating the impact of DZYX on apoptosis and autophagy in LLC cells. Furthermore, the anti-tumor mechanism of DZYX was explored through transcriptome research and detection of Akt, p-Akt, p-mTOR protein levels. Results: The ethanol extract of DZYX exhibited a concentration-dependent and time-dependent inhibitory effect on LLC cell viability, with an IC50 of 406.1 μg/ml. Moreover, the ethanol extract of DZYX significantly reduced the migration ability of LLC cells. Additionally, the alcoholic extract of DZYX upregulated the expression of Cleaved Caspase-3 and Beciln-1 proteins, while downregulating the expression of Bcl-2 in LLC cells. Importantly, DZYX ethanol extract down regulated the expression of Akt and p-mTOR proteins in LLC cells, which combined with transcriptome results indicated that the drug exerted a multi-target and multi-pathway effect, primarily related to inhibiting the activation of the PI3K/AKT/m-TOR signaling pathway. Conclusion: The alcoholic extract of DZYX demonstrates inhibitory effects on LLC cells, promoting apoptosis and autophagy. It is hypothesized that its anti-tumor mechanism is associated with the PI3K/AKT/m-TOR pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844023059200; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2023.e18712 |
| URL الوصول: | https://doaj.org/article/c9df0a2deb1d430c8686ccf9a92bce79 |
| رقم الأكسشن: | edsdoj.9df0a2deb1d430c8686ccf9a92bce79 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2023.e18712 |