دورية أكاديمية
Developing antibacterial peptides as a promising therapy for combating antibiotic-resistant Pseudomonas aeruginosa infections
| العنوان: | Developing antibacterial peptides as a promising therapy for combating antibiotic-resistant Pseudomonas aeruginosa infections |
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| المؤلفون: | Rula M. Darwish, Ali H. Salama |
| المصدر: | Veterinary World, Vol 17, Iss 6, Pp 1259-1264 (2024) |
| بيانات النشر: | Veterinary World, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | antimicrobial resistance, peptides, pseudomonas aeruginosa, synergism, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | Background and Aim: Antibiotic-resistant Pseudomonas aeruginosa poses a serious health threat. This study aimed to investigate the antibacterial activity of peptide KW-23 against drug-resistant P. aeruginosa and its potential for enhancing the efficacy of conventional antibiotics. Materials and Methods: KW-23 was synthesized from nine amino acids, specifically three tryptophans and three lysines. The purity of the substance was analyzed using reverse-phase high-performance liquid chromatography. The peptide was identified through mass spectrometry using electrospray ionization. The minimum inhibitory concentration (MIC) values of KW-23 in combination with conventional antibiotics against control and multidrug-resistant P. aeruginosa were determined utilizing broth microdilution. The erythrocyte hemolytic assay was used to measure toxicity. The KW-23 effect was analyzed using the time-kill curve. Results: The peptide exhibited strong antibacterial activity against control and multidrug-resistant strains of P. aeruginosa, with MICs of 4.5 μg/mL and 20 μg/mL, respectively. At higher concentration of 100 μg/mL, KW-23 exhibited a low hemolytic impact, causing no more than 3% damage to red blood. The cytotoxicity assay demonstrates KW-23’s safety, while the time-kill curve highlights its rapid and sustained antibacterial activity. The combination of KW-23 and gentamicin exhibited synergistic activity against both susceptible and resistant P. aeruginosa, with fractional inhibitory concentration index values of 0.07 and 0.27, respectively. Conclusion: The KW-23 synthesized in the laboratory significantly combats antibiotic-resistant P. aeruginosa. Due to its strong antibacterial properties and low toxicity to cells, KW-23 is a promising alternative to traditional antibiotics in combating multidrug-resistant bacteria. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0972-8988 2231-0916 |
| Relation: | https://www.veterinaryworld.org/Vol.17/June-2024/9.pdf; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.14202/vetworld.2024.1259-1264 |
| URL الوصول: | https://doaj.org/article/9e49a44b96114b339ae07c983e0cceaf |
| رقم الأكسشن: | edsdoj.9e49a44b96114b339ae07c983e0cceaf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 09728988 22310916 |
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| DOI: | 10.14202/vetworld.2024.1259-1264 |