دورية أكاديمية
Cloning of a new allele of ZmAMP1 and evaluation of its breeding value in hybrid maize
| العنوان: | Cloning of a new allele of ZmAMP1 and evaluation of its breeding value in hybrid maize |
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| المؤلفون: | Pingxi Wang, Yuan Yang, Dongdong Li, Jialiang Xu, Riliang Gu, Jun Zheng, Junjie Fu, Jianhua Wang, Hongwei Zhang |
| المصدر: | Crop Journal, Vol 11, Iss 1, Pp 157-165 (2023) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Agriculture LCC:Agriculture (General) |
| مصطلحات موضوعية: | Maize, Plant height, ZmAMP1, Green revolution, Agriculture, Agriculture (General), S1-972 |
| الوصف: | Gene resources associated with plant stature and flowering time are invaluable for maize breeding. In this study, using an F2:3 population derived from a natural semi-dwarf mutant grmm and a normal inbred line Si 273, we identified a major pleiotropic QTL on the distal long arm of chromosome 1 (qPH1_dla), and found that qPH1_dla controlled plant height, flowering time, ear and yield traits. qPH1_dla was fine-mapped to a 16 kb interval containing ZmAMP1, which was annotated as a glutamate carboxypeptidase. Allelism tests using two independent allelic mutants confirmed that ZmAMP1 was the causal gene. Real-time quantitative PCR and genomic sequence analysis suggested that a nonsynonymous mutation at the 598th base of ZmAMP1 gene was the causal sequence variant for the dwarfism of grmm. This novel ZmAMP1 allele was named ZmAMP1_grmm. RNA sequencing using two pairs of near isogenic lines (NILs) showed that 84 up-regulated and 68 down-regulated genes in dwarf NILs were enriched in 15 metabolic pathways. Finally, introgression of ZmAMP1_grmm into Zhengdan 958 and Xianyu 335 generated two improved F1 lines. In field tests, they were semi-dwarf, early-flowering, lodging-resistant, and high-yielding under high-density planting conditions, suggesting that ZmAMP1_grmm is a promising Green Revolution gene for maize hybrid breeding. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2214-5141 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2214514122001350; https://doaj.org/toc/2214-5141 |
| DOI: | 10.1016/j.cj.2022.06.001 |
| URL الوصول: | https://doaj.org/article/a9f30228b6724656a3d8b95393ee3641 |
| رقم الأكسشن: | edsdoj.9f30228b6724656a3d8b95393ee3641 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22145141 |
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| DOI: | 10.1016/j.cj.2022.06.001 |