دورية أكاديمية
Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera
| العنوان: | Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera |
|---|---|
| المؤلفون: | Cheng Chen, Yan Zhang, Wenhui Pi, Wenting Yang, Cunxi Nie, Jing Liang, Xi Ma, Wen-ju Zhang |
| المصدر: | AMB Express, Vol 9, Iss 1, Pp 1-10 (2019) |
| بيانات النشر: | SpringerOpen, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | Helicoverpa armigera, Cytochrome P450, CYP9A12, Gossypol, Detoxification, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract Gossypol is a toxic polyphenolic product that is derived from cotton plants. The toxicity of gossypol has limited the utilization of cottonseed meal (CSM) in the feed industry. The gene, Helicoverpa armigera CYP9A12, is a gossypol-inducible cytochrome P450 gene. The objective of our study was to obtain the functional recombinant H. armigera CYP9A12 enzyme in Pichia pastoris and to verify whether this candidate enzyme could decrease gossypol in vitro. Free and total gossypol contents were detected in the enzyme solution and in CSM. The H. armigera CYP9A12 enzyme degraded free concentration of gossypol. After optimization of the single-test and response surface method, free gossypol content could be decreased to 40.91 mg/kg in CSM by the H. armigera CYP9A12 enzyme when the initial temperature was 35 °C, the enzymatic hydrolysis time lasted 2.5 h, the enzyme addition was 2.5 mL, and the substrate moisture was 39%. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| Relation: | http://link.springer.com/article/10.1186/s13568-019-0823-4; https://doaj.org/toc/2191-0855 |
| DOI: | 10.1186/s13568-019-0823-4 |
| URL الوصول: | https://doaj.org/article/9fd0b9dcdbcc44159aa2a37cfe0acf1f |
| رقم الأكسشن: | edsdoj.9fd0b9dcdbcc44159aa2a37cfe0acf1f |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21910855 |
|---|---|
| DOI: | 10.1186/s13568-019-0823-4 |