دورية أكاديمية
أعرض في EDS

Elevation of MPF and MAPK gene expression, GSH content and mitochondrial distribution quality induced by melatonin promotes porcine oocyte maturation and development in vitro

التفاصيل البيبلوغرافية
العنوان: Elevation of MPF and MAPK gene expression, GSH content and mitochondrial distribution quality induced by melatonin promotes porcine oocyte maturation and development in vitro
المؤلفون: Zimo Zhao, Ling Yang, Dan Zhang, Zi Zheng, Ning Li, Qianjun Li, Maosheng Cui
المصدر: PeerJ, Vol 8, p e9913 (2020)
بيانات النشر: PeerJ Inc., 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine
LCC:Biology (General)
مصطلحات موضوعية: Porcine oocyte, Melatonin, MPF, MAPK, Maturation quality, Medicine, Biology (General), QH301-705.5
الوصف: The MPF and MAPK genes play crucial roles during oocyte maturation processes. However, the pattern of MPF and MAPK gene expression induced by melatonin (MT) and its correlation to oocyte maturation quality during the process of porcine oocyte maturation in vitro remains unexplored. To unravel it, in this study, we cultured the porcine oocytes in maturation medium supplemented with 0, 10−6, 10−9, and 10−12 mol/L melatonin. Later, we analyzed the MPF and MAPK gene expression levels by RT-PCR and determined the maturation index (survival and maturation rate of oocytes). The GSH content in the single oocyte, and cytoplasmic mitochondrial maturation distribution after porcine oocyte maturation in vitro was also evaluated. We also assessed the effects of these changes on parthenogenetic embryonic developmental potential. The oocytes cultured with 10−9mol/L melatonin concentration showed higher oocyte maturation rate, and MPF and MAPK genes expression levels along with better mitochondrial distribution than the 0, 10−6, and 10−12 mol/L melatonin concentrations (p
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2167-8359
Relation: https://peerj.com/articles/9913.pdf; https://peerj.com/articles/9913/; https://doaj.org/toc/2167-8359
DOI: 10.7717/peerj.9913
URL الوصول: https://doaj.org/article/9ffbf2989412475992f9e477ca135e56
رقم الأكسشن: edsdoj.9ffbf2989412475992f9e477ca135e56
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:21678359
DOI:10.7717/peerj.9913