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Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma

التفاصيل البيبلوغرافية
العنوان: Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma
المؤلفون: Hui-Ling Che, Doryn Meam-Yee Tan, Puvaneswari Meganathan, Yee-Lin Gan, Ghazali Abdul Razak, Ju-Yen Fu
المصدر: International Journal of Analytical Chemistry, Vol 2015 (2015)
بيانات النشر: Wiley, 2015.
سنة النشر: 2015
المجموعة: LCC:Analytical chemistry
مصطلحات موضوعية: Analytical chemistry, QD71-142
الوصف: Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in human plasma. Using both the external and internal standards, tocotrienol homologues were quantified via a normal-phase high performance liquid chromatography with fluorescence detector maintained at the excitation wavelength of 295 nm and the emission wavelength of 325 nm. The four tocotrienol homologues were well separated within 30 minutes. A large interindividual variation between subjects was observed as the absorption of tocotrienols is dependent on food matrix and gut lipolysis. The accuracies of lower and upper limit of quantification ranged between 92% and 109% for intraday assays and 90% and 112% for interday assays. This method was successfully applied to quantify the total amount of four tocotrienol homologues in human plasma.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1687-8760
1687-8779
Relation: https://doaj.org/toc/1687-8760; https://doaj.org/toc/1687-8779
DOI: 10.1155/2015/357609
URL الوصول: https://doaj.org/article/b020623857cc4bf09ef4866f03705d83
رقم الأكسشن: edsdoj.b020623857cc4bf09ef4866f03705d83
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:16878760
16878779
DOI:10.1155/2015/357609