دورية أكاديمية
Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
| العنوان: | Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model |
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| المؤلفون: | Chiara Liverani, Federico La Manna, Arwin Groenewoud, Laura Mercatali, Gabri Van Der Pluijm, Federica Pieri, Davide Cavaliere, Alessandro De Vita, Chiara Spadazzi, Giacomo Miserocchi, Alberto Bongiovanni, Federica Recine, Nada Riva, Dino Amadori, Ennio Tasciotti, Ewa Snaar-Jagalska, Toni Ibrahim |
| المصدر: | Biology Open, Vol 6, Iss 2, Pp 133-140 (2017) |
| بيانات النشر: | The Company of Biologists, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | Primary cultures, Collagen scaffolds, Zebrafish, Near-patient models, Science, Biology (General), QH301-705.5 |
| الوصف: | Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We present our data obtained on primary dedifferentiated liposarcoma cells cultured in a 3D scaffold-based system and injected into a zebrafish model. Primary cells were characterized in vitro for their morphological features, sensitivity to drugs and biomarker expression, and in vivo for their engraftment and invasiveness abilities. The 3D culture showed a higher enrichment in cancer cells than the standard monolayer culture and a better preservation of liposarcoma-associated markers. We also successfully grafted primary cells into zebrafish, showing their local migratory and invasive abilities. Our work provides proof of concept of the ability of 3D cultures to maintain the original phenotype of ex vivo cells, and highlights the potential of the zebrafish model to provide a versatile in vivo system for studies with limited biological material. Such models could be used in translational research studies for biomolecular analyses, drug screenings and tumor aggressiveness assays. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-6390 |
| Relation: | http://bio.biologists.org/content/6/2/133; https://doaj.org/toc/2046-6390 |
| DOI: | 10.1242/bio.022483 |
| URL الوصول: | https://doaj.org/article/b041616b805f44658bdba1de95196424 |
| رقم الأكسشن: | edsdoj.b041616b805f44658bdba1de95196424 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20466390 |
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| DOI: | 10.1242/bio.022483 |