دورية أكاديمية
DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids
| العنوان: | DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
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| المؤلفون: | Sebastian Canovas, Elena Ivanova, Raquel Romar, Soledad García-Martínez, Cristina Soriano-Úbeda, Francisco A García-Vázquez, Heba Saadeh, Simon Andrews, Gavin Kelsey, Pilar Coy |
| المصدر: | eLife, Vol 6 (2017) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | In vitro fertilization, blastocyst, pig, epigenetics, reproductive secretions, imprinting, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/23670; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.23670 |
| URL الوصول: | https://doaj.org/article/b0d9df1548bb433b96b4ab9ee2f95a58 |
| رقم الأكسشن: | edsdoj.b0d9df1548bb433b96b4ab9ee2f95a58 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.23670 |