دورية أكاديمية
Identification of a Novel Specific Cucurbitadienol Synthase Allele in Siraitia grosvenorii Correlates with High Catalytic Efficiency
| العنوان: | Identification of a Novel Specific Cucurbitadienol Synthase Allele in Siraitia grosvenorii Correlates with High Catalytic Efficiency |
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| المؤلفون: | Jing Qiao, Zuliang Luo, Zhe Gu, Yanling Zhang, Xindan Zhang, Xiaojun Ma |
| المصدر: | Molecules, Vol 24, Iss 3, p 627 (2019) |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | Siraitia grosvenorii, molecular breeding, site-directed mutant, mogrosides, cucurbitadienol synthase, single nucleotide polymorphism (SNP), Organic chemistry, QD241-441 |
| الوصف: | Mogrosides, the main bioactive compounds isolated from the fruits of Siraitia grosvenorii, are a group of cucurbitane-type triterpenoid glycosides that exhibit a wide range of notable biological activities and are commercially available worldwide as natural sweeteners. However, the extraction cost is high due to their relatively low contents in plants. Therefore, molecular breeding needs to be achieved when conventional plant breeding can hardly improve the quality so far. In this study, the levels of 21 active mogrosides and two precursors in 15 S. grosvenorii varieties were determined by HPLC-MS/MS and GC-MS, respectively. The results showed that the variations in mogroside V content may be caused by the accumulation of cucurbitadienol. Furthermore, a total of four wild-type cucurbitadienol synthase protein variants (50R573L, 50C573L, 50R573Q, and 50C573Q) based on two missense mutation single nucleotide polymorphism (SNP) sites were discovered. An in vitro enzyme reaction analysis indicated that 50R573L had the highest activity, with a specific activity of 10.24 nmol min−1 mg−1. In addition, a site-directed mutant, namely, 50K573L, showed a 33% enhancement of catalytic efficiency compared to wild-type 50R573L. Our findings identify a novel cucurbitadienol synthase allele correlates with high catalytic efficiency. These results are valuable for the molecular breeding of luohanguo. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1420-3049 24030627 |
| Relation: | https://www.mdpi.com/1420-3049/24/3/627; https://doaj.org/toc/1420-3049 |
| DOI: | 10.3390/molecules24030627 |
| URL الوصول: | https://doaj.org/article/b11e2d2fad774fcda7956afd2f8b094f |
| رقم الأكسشن: | edsdoj.b11e2d2fad774fcda7956afd2f8b094f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14203049 24030627 |
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| DOI: | 10.3390/molecules24030627 |