دورية أكاديمية
Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair
العنوان: | Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair |
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المؤلفون: | Ryota Chijimatsu, Makoto Ikeya, Yukihiko Yasui, Yasutoshi Ikeda, Kosuke Ebina, Yu Moriguchi, Kazunori Shimomura, David A Hart, Yoshikawa Hideki, Nakamura Norimasa |
المصدر: | Stem Cells International, Vol 2017 (2017) |
بيانات النشر: | Wiley, 2017. |
سنة النشر: | 2017 |
المجموعة: | LCC:Internal medicine |
مصطلحات موضوعية: | Internal medicine, RC31-1245 |
الوصف: | Mesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs) are a promising cell source for the repair of skeletal disorders. Recently, neural crest cells (NCCs) were reported to be effective for inducing mesenchymal progenitors, which have potential to differentiate into osteochondral lineages. Our aim was to investigate the feasibility of MSC-like cells originated from iPSCs via NCCs for osteochondral repair. Initially, MSC-like cells derived from iPSC-NCCs (iNCCs) were generated and characterized in vitro. These iNCC-derived MSC-like cells (iNCMSCs) exhibited a homogenous population and potential for osteochondral differentiation. No upregulation of pluripotent markers was detected during culture. Second, we implanted iNCMSC-derived tissue-engineered constructs into rat osteochondral defects without any preinduction for specific differentiation lineages. The implanted cells remained alive at the implanted site, whereas they failed to repair the defects, with only scarce development of osteochondral tissue in vivo. With regard to tumorigenesis, the implanted cells gradually disappeared and no malignant cells were detected throughout the 2-month follow-up. While this study did not show that iNCMSCs have efficacy for repair of osteochondral defects when implanted under undifferentiated conditions, iNCMSCs exhibited good chondrogenic potential in vitro under appropriate conditions. With further optimization, iNCMSCs may be a new source for tissue engineering of cartilage. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1687-966X 1687-9678 |
Relation: | https://doaj.org/toc/1687-966X; https://doaj.org/toc/1687-9678 |
DOI: | 10.1155/2017/1960965 |
URL الوصول: | https://doaj.org/article/eb1360a30c8c44f180b3dfaabd9eba71 |
رقم الأكسشن: | edsdoj.b1360a30c8c44f180b3dfaabd9eba71 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 1687966X 16879678 |
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DOI: | 10.1155/2017/1960965 |