دورية أكاديمية
Tailoring the stress response of human skin cells by substantially limiting the nuclear localization of angiogenin
| العنوان: | Tailoring the stress response of human skin cells by substantially limiting the nuclear localization of angiogenin |
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| المؤلفون: | Rosanna Culurciello, Ilaria Di Nardo, Andrea Bosso, Francesca Tortora, Romualdo Troisi, Filomena Sica, Angela Arciello, Eugenio Notomista, Elio Pizzo |
| المصدر: | Heliyon, Vol 10, Iss 3, Pp e24556- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Stress induced RNases, Stress granules, Cell homeostasis, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Human angiogenin (hANG) is the most studied stress-induced ribonuclease (RNase). In physiological conditions it performs its main functions in nucleoli, promoting cell proliferation by rDNA transcription, whereas it is strongly limited by its inhibitor (RNH1) throughout the rest of the cell. In stressed cells hANG dissociates from RNH1 and thickens in the cytoplasm where it manages the translational arrest and the recruitment of stress granules, thanks to its propensity to cleave tRNAs and to induce the release of active halves. Since it exists a clear connection between hANG roles and its intracellular routing, starting from our recent findings on heterologous ANG (ANG) properties in human keratinocytes (HaCaT cells), here we designed a variant unable to translocate into the nucleus with the aim of thoroughly verifying its potentialities under stress. This variant, widely characterized for its structural features and biological attitudes, shows more pronounced aid properties than unmodified protein. The collected evidence thus fully prove that ANG stress-induced skills in assisting cellular homeostasis are strictly due to its cytosolic localization. This study opens an interesting scenario for future studies regarding both the strengthening of skin defences and in understanding the mechanism of action of these special enzymes potentially suitable for any cell type. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844024005875; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2024.e24556 |
| URL الوصول: | https://doaj.org/article/b23113ca23a04c4199aeae3934dd997b |
| رقم الأكسشن: | edsdoj.b23113ca23a04c4199aeae3934dd997b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2024.e24556 |