دورية أكاديمية
Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation
| العنوان: | Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation |
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| المؤلفون: | Juan J. A. M. Muñoz, Rafael Dariolli, Caio Mateus da Silva, Elida A. Neri, Iuri C. Valadão, Lauro Thiago Turaça, Vanessa M. Lima, Mariana Lombardi Peres de Carvalho, Mariliza R. Velho, Eric A. Sobie, Jose E. Krieger |
| المصدر: | Stem Cell Research & Therapy, Vol 13, Iss 1, Pp 1-27 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Cardiac differentiation, hiPSC-CM, Time-dependent regulated transcripts, miRNA, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are a promising disease model, even though hiPSC-CMs cultured for extended periods display an undifferentiated transcriptional landscape. MiRNA–target gene interactions contribute to fine-tuning the genetic program governing cardiac maturation and may uncover critical pathways to be targeted. Methods We analyzed a hiPSC-CM public dataset to identify time-regulated miRNA–target gene interactions based on three logical steps of filtering. We validated this process in silico using 14 human and mouse public datasets, and further confirmed the findings by sampling seven time points over a 30-day protocol with a hiPSC-CM clone developed in our laboratory. We then added miRNA mimics from the top eight miRNAs candidates in three cell clones in two different moments of cardiac specification and maturation to assess their impact on differentiation characteristics including proliferation, sarcomere structure, contractility, and calcium handling. Results We uncovered 324 interactions among 29 differentially expressed genes and 51 miRNAs from 20,543 transcripts through 120 days of hiPSC-CM differentiation and selected 16 genes and 25 miRNAs based on the inverse pattern of expression (Pearson R-values |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-022-03138-x |
| URL الوصول: | https://doaj.org/article/b25de1df4cea49079927edc93026a79a |
| رقم الأكسشن: | edsdoj.b25de1df4cea49079927edc93026a79a |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-022-03138-x |