Surplus neonatal dairy calves are routinely transported long distances from the dairy farm of birth to calf raisers. Most of the research assessing the effect of transportation on young calves has focused on physiology and health, without considering calf affective states. The aim of this study was to assess the affective response of young male dairy calves to transportation using conditioned place aversion (CPA) testing. Three bouts of transportation exposed dairy calves (n = 95) to transportation for 6 (n = 31), 12 (n = 32), or 16 h (n = 30). During transportation, the trailer interior was made visually distinct using blue horizontal (n = 2 groups) or red vertical (n = 1 group) stripes. Distinct color and pattern combinations were used to create a visual association with transportation. A subset of calves (6 h = 14; 12 h = 14; 16 h = 14) were selected for CPA testing if they were ≥7 and
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