دورية أكاديمية
In Vitro Activity of Quaternary Ammonium in Prototheca Isolated from Clinical Bovine Mastitis Identified by Mass Spectrometry and PCR Sequencing of the cytb Gene Marker
| العنوان: | In Vitro Activity of Quaternary Ammonium in Prototheca Isolated from Clinical Bovine Mastitis Identified by Mass Spectrometry and PCR Sequencing of the cytb Gene Marker |
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| المؤلفون: | Marcelo Fagali Arabe Filho, Tomasz Jagielski, Angelika Proskurnicka, Marcos Veiga dos Santos, Carlos Eduardo Fidelis, Felipe Freitas Guimarães, Simony Trevizan Guerra, Sâmea Fernandes Joaquim, Mateus de Souza Ribeiro Mioni, José Carlos de Figueiredo Pantoja, Helio Langoni, Luísa Fernanda García Sanchez, Marcio Garcia Ribeiro |
| المصدر: | Animals, Vol 13, Iss 20, p 3286 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Veterinary medicine LCC:Zoology |
| مصطلحات موضوعية: | Prototheca bovis, algaecide compounds, intramammary protothecosis, molecular typing, clinical severity scoring, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| الوصف: | The in vitro algaecide activity of quaternary ammonium (QA) against Prototheca isolated from bovine clinical mastitis was investigated, in which the clinical severity was scored, milk samples were subjected to microbiological culture, and algal species were identified by molecular typing. A total of 4275 milk clinical samples of different cows from ten large dairy farms were used. Forty-four (1%) samples of cows from three dairy farms yielded growth of Prototheca, of which 88.6% (39/44) were identified as Prototheca bovis and 11.3% (5/44) as Prototheca sp. by MALDI-TOF MS, whereas 100% of the isolates were identified as P. bovis using PCR sequencing of the cytb gene. Among cows for which clinical severity scoring was available, 78.8% (26/33) and 21.2% (7/33) had mild and moderate infections, respectively, whereas no animal showed severe clinical signs. The algaecide activity of QA in Prototheca was observed in low concentrations among all isolates, in 20.4% (9/44) at 35 ppm, 36.4% (16/44) at 17 ppm, and 43.2% (19/44) at an 8 ppm, in addition to activity on three reference Prototheca strains. Overall, the study highlights the predominance of P. bovis as the causative agent of algal mastitis in bovines. Prototheca induced abnormalities preponderantly in the milk and mammary gland tissue of cows, and to our knowledge, our study is the first to apply clinical severity scoring in protothecal mastitis. In addition, the study underlines the activity of QA in low concentrations against Prototheca, indicating its potential use as an antiseptic/disinfectant in milking facilities and dairy environments. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/13/20/3286; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani13203286 |
| URL الوصول: | https://doaj.org/article/b29502fc9bc44293a86bb0c848df802d |
| رقم الأكسشن: | edsdoj.b29502fc9bc44293a86bb0c848df802d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20762615 |
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| DOI: | 10.3390/ani13203286 |