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Tetraspanin 8-Rictor-Integrin α3 Complex Is Required for Glioma Cell Migration

التفاصيل البيبلوغرافية
العنوان: Tetraspanin 8-Rictor-Integrin α3 Complex Is Required for Glioma Cell Migration
المؤلفون: Si-Jian Pan, Shi-Kun Zhan, Yi-Xin Pan, Wei Liu, Liu-Guan Bian, Bomin Sun, Qing-Fang Sun
المصدر: International Journal of Molecular Sciences, Vol 16, Iss 3, Pp 5363-5374 (2015)
بيانات النشر: MDPI AG, 2015.
سنة النشر: 2015
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: malignant glioma, tetraspanin 8, integrin α3, rictor, mTORC2, AKT and cell migration, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: The malignant glioma remains one of the most aggressive human malignancies with extremely poor prognosis. Glioma cell invasion and migration are the main causes of death. In the current study, we studied the expression and the potential functions of tetraspanin 8 (Tspan8) in malignant gliomas. We found that Tspan8 expression level is high in both malignant glioma tissues and in several human glioma cell lines, where it formed a complex integrin α3 and rictor, the latter is a key component of mammalian target of rapamycin (mTOR) complex 2 (mTORC2). Disruption of this complex, through siRNA-mediated knockdown of anyone of these three proteins, inhibited U251MG glioma cell migration in vitro. We further showed that Tspan8-rictor association appeared required for mTORC2 activation. Knockdown of Tspan8 by the targeted siRNAs prevented mTOR-rictor (mTORC2) assembly as well as phosphorylation of AKT (Ser-473) and protein kinase C α (PKCα) in U251MG cells. Together, these results demonstrate that over-expressed Tspan8 in malignant glioma forms a complex with rictor and integrin α3 to mediate mTORC2 activation and glioma cell migration. Therefore, targeting Tspan8-rictor-integrin α3 complex may provide a potential therapeutic intervention for malignant glioma.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/16/3/5363; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms16035363
URL الوصول: https://doaj.org/article/cb2a0359ff434d229c2101d265024fa4
رقم الأكسشن: edsdoj.b2a0359ff434d229c2101d265024fa4
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms16035363