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MicroRNA-29 facilitates transplantation of bone marrow-derived mesenchymal stem cells to alleviate pelvic floor dysfunction by repressing elastin

التفاصيل البيبلوغرافية
العنوان: MicroRNA-29 facilitates transplantation of bone marrow-derived mesenchymal stem cells to alleviate pelvic floor dysfunction by repressing elastin
المؤلفون: Minfei Jin, Yuelin Wu, Jun Wang, Weiping Ye, Lei Wang, Peipei Yin, Wei Liu, Chenhao Pan, Xiaolin Hua
المصدر: Stem Cell Research & Therapy, Vol 7, Iss 1, Pp 1-9 (2016)
بيانات النشر: BMC, 2016.
سنة النشر: 2016
المجموعة: LCC:Medicine (General)
LCC:Biochemistry
مصطلحات موضوعية: Pelvic floor dysfunction (PFD), Bone marrow-derived mesenchymal stem cells (BMSC), Elastin, MicroRNA-29a-3p, Nanoparticles, Medicine (General), R5-920, Biochemistry, QD415-436
الوصف: Abstract Background Pelvic floor dysfunction (PFD) is a condition affecting many women worldwide, with symptoms including stress urinary incontinence (SUI) and pelvic organ prolapse (POP). We have previously demonstrated stable elastin-expressing bone marrow-derived mesenchymal stem cells (BMSCs) attenuated PFD in rats, and aim to further study the effect of microRNA-29a-3p regulation on elastin expression and efficacy of BMSC transplantation therapy. Methods We inhibited endogenous microRNA-29a-3p in BMSCs and investigated its effect on elastin expression by RT-PCR and Western blot. MicroRNA-29-inhibited BMSCs were then transplanted into PFD rats, accompanied by sustained release of bFGF using formulated bFGF in poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NP), followed by evaluation of urodynamic tests. Results MicroRNA-29a-3p inhibition resulted in upregulated expression and secretion of elastin in in vitro culture of BMSCs. After co-injection with PLGA-loaded bFGF NP into the PFD rats in vivo, microRNA-29a-3p-inhibited BMSCs significantly improved the urodynamic test results. Conclusions Our multidisciplinary study, combining microRNA biology, genetically engineered BMSCs, and nanoparticle technology, provides an excellent stem cell-based therapy for repairing connective tissues and treating PFD.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1757-6512
Relation: http://link.springer.com/article/10.1186/s13287-016-0428-7; https://doaj.org/toc/1757-6512
DOI: 10.1186/s13287-016-0428-7
URL الوصول: https://doaj.org/article/b2da0ad9f3a84651af9f0b9e5a09b10e
رقم الأكسشن: edsdoj.b2da0ad9f3a84651af9f0b9e5a09b10e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17576512
DOI:10.1186/s13287-016-0428-7