دورية أكاديمية
Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction
| العنوان: | Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction |
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| المؤلفون: | Maria Michela Cesare, Francesca Felice, Veronica Conti, Luca Cerri, Ylenia Zambito, Marco Romi, Giampiero Cai, Claudio Cantini, Rossella Di Stefano |
| المصدر: | Molecules, Vol 26, Iss 14, p 4289 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | tomato peel extract, tomato by-products, oxidative stress, drought stress, polyphenols, human vascular endothelial cells, Organic chemistry, QD241-441 |
| الوصف: | Background: Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso di Pitigliano (RED), obtained by growing plants in normal conditions (-Ctr) or in drought stress conditions (-Ds) for their beneficial effects on vascular related dysfunction. Methods: The antioxidant activity and total polyphenol content (TPC) were measured. The identification of bioactive compounds of tomato peel was performed by HPLC. HUVEC were pre-treated with different TPC of RED-Ctr or RED-Ds, then stressed with H2O2. Cell viability, ROS production and CAT, SOD and GPx activities were evaluated. Permeation of antioxidant molecules contained in RED across excised rat intestine was also studied. Results: RED-Ds tomato peel extract possessed higher TPC than compared to RED-Ctr (361.32 ± 7.204 mg vs. 152.46 ± 1.568 mg GAE/100 g fresh weight). All extracts were non-cytotoxic. Two hour pre-treatment with 5 µg GAE/mL from RED-Ctr or RED-Ds showed protection from H2O2-induced oxidative stress and significantly reduced ROS production raising SOD and CAT activity (* p < 0.05 and ** p < 0.005 vs. H2O2, respectively). The permeation of antioxidant molecules contained in RED-Ctr or RED-Ds across excised rat intestine was high with non-significant difference between the two RED types (41.9 ± 9.6% vs. 26.6 ± 7.8%). Conclusions: RED-Ds tomato peel extract represents a good source of bioactive molecules, which protects HUVECs from oxidative stress at low concentration. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1420-3049 72277548 |
| Relation: | https://www.mdpi.com/1420-3049/26/14/4289; https://doaj.org/toc/1420-3049 |
| DOI: | 10.3390/molecules26144289 |
| URL الوصول: | https://doaj.org/article/b2e13e72277548f6b43355718fc5e3dc |
| رقم الأكسشن: | edsdoj.b2e13e72277548f6b43355718fc5e3dc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14203049 72277548 |
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| DOI: | 10.3390/molecules26144289 |