دورية أكاديمية
A novel protein derived from lamprey supraneural body tissue with efficient cytocidal actions against tumor cells
| العنوان: | A novel protein derived from lamprey supraneural body tissue with efficient cytocidal actions against tumor cells |
|---|---|
| المؤلفون: | Yue Pang, Changzhi Li, Shiyue Wang, Wei Ba, Tao Yu, Guangying Pei, Dan Bi, Hongfang Liang, Xiong Pan, Ting Zhu, Meng Gou, Yinglun Han, Qingwei Li |
| المصدر: | Cell Communication and Signaling, Vol 15, Iss 1, Pp 1-16 (2017) |
| بيانات النشر: | BMC, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine LCC:Cytology |
| مصطلحات موضوعية: | Lamprey, LIP, Cytotoxic activity, Inflammatory, Phosphatidylserine, Medicine, Cytology, QH573-671 |
| الوصف: | Abstract Background In previous research, we found that cell secretion from the adult lamprey supraneural body tissues possesses cytocidal activity against tumor cells, but the protein with cytocidal activity was unidentified. Methods A novel lamprey immune protein (LIP) as defense molecule was first purified and identified in jawless vertebrates (cyclostomes) using hydroxyapatite column and Q Sepharose Fast Flow column. After LIP stimulation, morphological changes of tumor cells were analysed and measured whether in vivo or in vitro. Results LIP induces remarkable morphological changes in tumor cells, including cell blebbing, cytoskeletal alterations, mitochondrial fragmentation and endoplasmic reticulum vacuolation, and most of the cytoplasmic and organelle proteins are released following treatment with LIP. LIP evokes an elevation of intracellular calcium and inflammatory molecule levels. Our analysis of the cytotoxic mechanism suggests that LIP can upregulate the expression of caspase 1, RIPK1, RIP3 to trigger pyroptosis and necroptosis. To examine the effect of LIP in vivo, tumor xenograft experiments were performed, and the results indicated that LIP inhibits tumor growth without damage to mice. In addition, the cytotoxic action of LIP depended on the phosphatidylserine (PS) content of the cell membrane. Conclusions These observations suggest that LIP plays a crucial role in tumor cell survival and growth. The findings will also help to elucidate the mechanisms of host defense in lamprey. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1478-811X |
| Relation: | http://link.springer.com/article/10.1186/s12964-017-0198-6; https://doaj.org/toc/1478-811X |
| DOI: | 10.1186/s12964-017-0198-6 |
| URL الوصول: | https://doaj.org/article/b36d744db34c4efe8248a3eb1864f0aa |
| رقم الأكسشن: | edsdoj.b36d744db34c4efe8248a3eb1864f0aa |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1478811X |
|---|---|
| DOI: | 10.1186/s12964-017-0198-6 |