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HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells

التفاصيل البيبلوغرافية
العنوان: HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells
المؤلفون: Shiqian Li, Yafei Wang, Miesje van der Stoel, Xin Zhou, Shrinidhi Madhusudan, Kristiina Kanerva, Van Dien Nguyen, Nazli Eskici, Vesa M. Olkkonen, You Zhou, Taneli Raivio, Elina Ikonen
المصدر: Genome Biology, Vol 25, Iss 1, Pp 1-26 (2024)
بيانات النشر: BMC, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
LCC:Genetics
مصطلحات موضوعية: Auxin-inducible degron 2, Homology-directed repair enhancers, Homozygous knock-in, Functional inactivation, Chemogenetics, Human embryonic stem cell (hESC), Biology (General), QH301-705.5, Genetics, QH426-470
الوصف: Abstract Recent developments in auxin-inducible degron (AID) technology have increased its popularity for chemogenetic control of proteolysis. However, generation of human AID cell lines is challenging, especially in human embryonic stem cells (hESCs). Here, we develop HiHo-AID2, a streamlined procedure for rapid, one-step generation of human cancer and hESC lines with high homozygous degron-tagging efficiency based on an optimized AID2 system and homology-directed repair enhancers. We demonstrate its application for rapid and inducible functional inactivation of twelve endogenous target proteins in five cell lines, including targets with diverse expression levels and functions in hESCs and cells differentiated from hESCs.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1474-760X
Relation: https://doaj.org/toc/1474-760X
DOI: 10.1186/s13059-024-03187-w
URL الوصول: https://doaj.org/article/b386292dc07c45e88b124344bcaa5ff8
رقم الأكسشن: edsdoj.b386292dc07c45e88b124344bcaa5ff8
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1474760X
DOI:10.1186/s13059-024-03187-w