دورية أكاديمية
N-Terminal Amino Acid Affects the Translation Efficiency at Lower Temperatures in a Reconstituted Protein Synthesis System
| العنوان: | N-Terminal Amino Acid Affects the Translation Efficiency at Lower Temperatures in a Reconstituted Protein Synthesis System |
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| المؤلفون: | Tomoe Fuse-Murakami, Rena Matsumoto, Takashi Kanamori |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 10, p 5264 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | cell-free protein synthesis, PURE system, N-terminal sequence, translation efficiency, early elongation, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | The Escherichia coli (E. coli)-based protein synthesis using recombinant elements (PURE) system is a cell-free protein synthesis system reconstituted from purified factors essential for E. coli translation. The PURE system is widely used for basic and synthetic biology applications. One of the major challenges associated with the PURE system is that the protein yield of the system varies depending on the protein. Studies have reported that the efficiency of translation is significantly affected by nucleotide and amino acid sequences, especially in the N-terminal region. Here, we investigated the inherent effect of various N-terminal sequences on protein synthesis using the PURE system. We found that a single amino acid substitution in the N-terminal region significantly altered translation efficiency in the PURE system, especially at low temperatures. This result gives us useful suggestions for the expression of the protein of interest in vitro and in vivo. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 25105264 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/10/5264; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25105264 |
| URL الوصول: | https://doaj.org/article/b3920f8ca7a34eb59b881325a1809fd7 |
| رقم الأكسشن: | edsdoj.b3920f8ca7a34eb59b881325a1809fd7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25105264 14220067 16616596 |
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| DOI: | 10.3390/ijms25105264 |