دورية أكاديمية
Heat induces end to end repetitive association in P. furiosus l-asparaginase which enables its thermophilic property
العنوان: | Heat induces end to end repetitive association in P. furiosus l-asparaginase which enables its thermophilic property |
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المؤلفون: | Pankaj Sharma, Rachana Tomar, Shivpratap Singh Yadav, Maulik D. Badmalia, Samir Kumar Nath, Ashish, Bishwajit Kundu |
المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
بيانات النشر: | Nature Portfolio, 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Medicine LCC:Science |
مصطلحات موضوعية: | Medicine, Science |
الوصف: | Abstract It remains undeciphered how thermophilic enzymes display enhanced stability at elevated temperatures. Taking l-asparaginase from P. furiosus (PfA) as an example, we combined scattering shapes deduced from small-angle X-ray scattering (SAXS) data at increased temperatures with symmetry mates from crystallographic structures to find that heating caused end-to-end association. The small contact point of self-binding appeared to be enabled by a terminal short β-strand in N-terminal domain, Leu179-Val-Val-Asn182 (LVVN). Interestingly, deletion of this strand led to a defunct enzyme, whereas suplementation of the peptide LVVN to the defunct enzyme restored structural frameworkwith mesophile-type functionality. Crystal structure of the peptide-bound defunct enzyme showed that one peptide ispresent in the same coordinates as in original enzyme, explaining gain-of lost function. A second peptide was seen bound to the protein at a different location suggesting its possible role in substrate-free molecular-association. Overall, we show that the heating induced self-assembly of native shapes of PfA led to an apparent super-stable assembly. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2045-2322 |
Relation: | https://doaj.org/toc/2045-2322 |
DOI: | 10.1038/s41598-020-78877-z |
URL الوصول: | https://doaj.org/article/cb4ab17049d744219a90d1b286d8a31c |
رقم الأكسشن: | edsdoj.b4ab17049d744219a90d1b286d8a31c |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 20452322 |
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DOI: | 10.1038/s41598-020-78877-z |