دورية أكاديمية
Properties of Two Broad Host Range Phages of Yersinia enterocolitica Isolated from Wild Animals
العنوان: | Properties of Two Broad Host Range Phages of Yersinia enterocolitica Isolated from Wild Animals |
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المؤلفون: | Jens A. Hammerl, Andrea Barac, Philipp Erben, Julius Fuhrmann, Ashish Gadicherla, Franziska Kumsteller, Anne Lauckner, Felix Müller, Stefan Hertwig |
المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 21, p 11381 (2021) |
بيانات النشر: | MDPI AG, 2021. |
سنة النشر: | 2021 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | Yersinia enterocolitica, phage, genome, application, therapy, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | Yersinia (Y.) enterocolitica and Y. pseudotuberculosis are important zoonotic agents which can infect both humans and animals. To combat these pathogens, the application of strictly lytic phages may be a promising tool. Since only few Yersinia phages have been described yet, some of which demonstrated a high specificity for certain serotypes, we isolated two phages from game animals and characterized them in terms of their morphology, host specificity, lytic activity on two bio-/serotypes and genome composition. The T7-related podovirus vB_YenP_Rambo and the myovirus vB_YenM_P281, which is very similar to a previously described phage PY100, showed a broad host range. Together, they lysed all the 62 tested pathogenic Y. enterocolitica strains belonging to the most important bio-/serotypes in Europe. A cocktail containing these two phages strongly reduced cultures of a bio-/serotype B4/O:3 and a B2/O:9 strain, even at very low MOIs (multiplicity of infection) and different temperatures, though, lysis of bio-/serotype B2/O:9 by vB_YenM_P281 and also by the related phage PY100 only occurred at 37 °C. Both phages were additionally able to lyse various Y. pseudotuberculosis strains at 28 °C and 37 °C, but only when the growth medium was supplemented with calcium and magnesium cations. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 1661-6596 |
Relation: | https://www.mdpi.com/1422-0067/22/21/11381; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
DOI: | 10.3390/ijms222111381 |
URL الوصول: | https://doaj.org/article/ab4da8c01a024268a1b9daaf484ddeb6 |
رقم الأكسشن: | edsdoj.b4da8c01a024268a1b9daaf484ddeb6 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14220067 16616596 |
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DOI: | 10.3390/ijms222111381 |