دورية أكاديمية
The Anti-Melanoma Effect of Betulinic Acid Functionalized Gold Nanoparticles: A Mechanistic In Vitro Approach
العنوان: | The Anti-Melanoma Effect of Betulinic Acid Functionalized Gold Nanoparticles: A Mechanistic In Vitro Approach |
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المؤلفون: | Roxana Ghiulai, Alexandra Mioc, Roxana Racoviceanu, Marius Mioc, Andreea Milan, Alexandra Prodea, Alexandra Semenescu, Cristina Dehelean, Lucian Barbu Tudoran, Ștefana Avram, Cristina Trandafirescu, Codruța Șoica |
المصدر: | Pharmaceuticals, Vol 15, Iss 11, p 1362 (2022) |
بيانات النشر: | MDPI AG, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Medicine LCC:Pharmacy and materia medica |
مصطلحات موضوعية: | betulinic acid, gold nanoparticles, melanoma, apoptosis, mitochondria, protein expression, Medicine, Pharmacy and materia medica, RS1-441 |
الوصف: | Implementing metallic nanoparticles as research instruments for the transport of therapeutically active compounds remains a fundamentally vital work direction that can still potentially generate novelties in the field of drug formulation development. Gold nanoparticles (GNP) are easily tunable carriers for active phytocompounds like pentacyclic triterpenes. These formulations can boost the bioavailability of a lipophilic structure and, in some instances, can also enhance its therapeutic efficacy. In our work, we proposed a biological in vitro assessment of betulinic acid (BA)-functionalized GNP. BA-GNP were obtained by grafting BA onto previously synthesized citrate-capped GNP through the use of cysteamine as a linker. The nanoformulation was tested in HaCaT human keratinocytes and RPMI-7951 human melanoma cells, revealing selective cytotoxic properties and stronger antiproliferative effects compared to free BA. Further examinations revealed a pro-apoptotic effect, as evidenced by morphological changes in melanoma cells and supported by western blot data showing the downregulation of anti-apoptotic Bcl-2 expression coupled with the upregulation of pro-apoptotic Bax. GNP also significantly inhibited mitochondrial respiration, confirming its mitochondrial-targeted activity. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1424-8247 |
Relation: | https://www.mdpi.com/1424-8247/15/11/1362; https://doaj.org/toc/1424-8247 |
DOI: | 10.3390/ph15111362 |
URL الوصول: | https://doaj.org/article/b4ebc9b1a2a8493fb0c4ffe3b1189984 |
رقم الأكسشن: | edsdoj.b4ebc9b1a2a8493fb0c4ffe3b1189984 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14248247 |
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DOI: | 10.3390/ph15111362 |