دورية أكاديمية
Evaluation of bacterial uptake, antibacterial efficacy against Escherichia coli, and cytotoxic effects of moxifloxacin-loaded solid lipid nanoparticles
| العنوان: | Evaluation of bacterial uptake, antibacterial efficacy against Escherichia coli, and cytotoxic effects of moxifloxacin-loaded solid lipid nanoparticles |
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| المؤلفون: | Kiymaci Merve Eylul, Topal Gizem Ruya, Esim Ozgur, Bacanli Merve, Ozkan Cansel Kose, Erdem Onur, Savaser Ayhan, Ozkan Yalcin |
| المصدر: | Arhiv za Higijenu Rada i Toksikologiju, Vol 73, Iss 4, Pp 260-269 (2022) |
| بيانات النشر: | Sciendo, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Toxicology. Poisons |
| مصطلحات موضوعية: | antibiotic, biocompatibility, drug resistance, e. coli, lipid-based nanoparticles, raw 264.7 cell line, antibiotik, biokompatibilnost, nanočestice s lipidima, rezistentnost na lijek, stanična linija raw 264.7, Toxicology. Poisons, RA1190-1270 |
| الوصف: | Moxifloxacin (MOX) is an important antibiotic commonly used in the treatment of recurrent Escherichia coli (E. coli) infections. The aim of this study was to investigate its antibacterial efficiency when used with solid lipid nanoparticles (SNLs) and nanostructured lipid carriers (NLCs) as delivery vehicles. For this purpose we designed two SLNs (SLN1 and SLN2) and two NLCs (NLC1 and NLC2) of different characteristics (particle size, size distribution, zeta potential, and encapsulation efficiency) and loaded them with MOX to determine its release, antibacterial activity against E. coli, and their cytotoxicity to the RAW 264.7 monocyte/macrophage-like cell line in vitro. With bacterial uptake of 57.29 %, SLN1 turned out to be significantly more effective than MOX given as standard solution, whereas SLN2, NLC1, and NLC2 formulations with respective bacterial uptakes of 50.74 %, 39.26 %, and 32.79 %, showed similar activity to standard MOX. Cytotoxicity testing did not reveal significant toxicity of nanoparticles, whether MOX-free or MOX-loaded, against RAW 264.7 cells. Our findings may show the way for a development of effective lipid carriers that reduce side effects and increase antibacterial treatment efficacy in view of the growing antibiotic resistance. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Croatian |
| تدمد: | 1848-6312 |
| Relation: | https://doaj.org/toc/1848-6312 |
| DOI: | 10.2478/aiht-2022-73-3667 |
| URL الوصول: | https://doaj.org/article/b5cb5e196af9429ebc0192ac532f1c60 |
| رقم الأكسشن: | edsdoj.b5cb5e196af9429ebc0192ac532f1c60 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18486312 |
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| DOI: | 10.2478/aiht-2022-73-3667 |