دورية أكاديمية
Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil
| العنوان: | Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil |
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| المؤلفون: | Jadriane de Almeida Xavier, Iara Barros Valentim, Fabiana O. S. Camatari, Alberto M. M. de Almeida, Henrique Fonseca Goulart, Jamylle Nunes de Souza Ferro, Emiliano de Oliveira Barreto, Bruno Coelho Cavalcanti, Carla B. G. Bottoli, Marília Oliveira Fonseca Goulart |
| المصدر: | Pharmaceutical Biology, Vol 55, Iss 1, Pp 1884-1893 (2017) |
| بيانات النشر: | Taylor & Francis Group, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | biologically active phenols, chemical profile, content of organic volatiles, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Context: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil – sample A from Ribeira do Pombal and B, from Tucano – were investigated, with new information regarding their biological activities. Objective: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples. Material and methods: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH•, FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0.04–25 μg/mL concentrations, for 72 h. The determination of primary phenols by ultra high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) and volatile organic compounds content by gas chromatography-mass spectrometry (GC-MS) were also performed. Results: The EEP polar fractions exhibited up to 90% protection against lipid peroxidation. The IC50 value for anti-glycation activity of EEP was between 16.5 and 19.2 μg/mL, close to aminoguanidine (IC50 = 7.7 μg/mL). The use of UHPLC-MS/MS and GC-MS allowed the identification of 12 bioactive phenols in the EEP and 24 volatile compounds, all already reported. Conclusions: The samples present good antioxidant/anti-glycation/cytotoxic activities and a plethora of biologically active compounds. These results suggest a potential role of propolis in targeting ageing and diseases associated with oxidative and carbonylic stress, aggregating value to them. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1388-0209 1744-5116 13880209 |
| Relation: | https://doaj.org/toc/1388-0209; https://doaj.org/toc/1744-5116 |
| DOI: | 10.1080/13880209.2017.1340962 |
| URL الوصول: | https://doaj.org/article/db5eb7c4284f47b08443334f23c1ec4e |
| رقم الأكسشن: | edsdoj.b5eb7c4284f47b08443334f23c1ec4e |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 13880209 17445116 |
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| DOI: | 10.1080/13880209.2017.1340962 |