دورية أكاديمية

Global analysis of common bean multidrug and toxic compound extrusion transporters (PvMATEs): PvMATE8 and pinto bean seed coat darkening

التفاصيل البيبلوغرافية
العنوان: Global analysis of common bean multidrug and toxic compound extrusion transporters (PvMATEs): PvMATE8 and pinto bean seed coat darkening
المؤلفون: Nishat S. Islam, Kishor Duwadi, Ling Chen, Aga Pajak, Tim McDowell, Frédéric Marsolais, Sangeeta Dhaubhadel
المصدر: Frontiers in Plant Science, Vol 13 (2022)
بيانات النشر: Frontiers Media S.A., 2022.
سنة النشر: 2022
المجموعة: LCC:Plant culture
مصطلحات موضوعية: common bean, proanthocyanidin transporter, seed coat darkening, MATE (multidrug and toxic compound extrusion), epicatechin 3’-O-glucoside, Plant culture, SB1-1110
الوصف: In common bean (Phaseolus vulgaris L.), postharvest seed coat darkening is an undesirable trait that affects crop value. The increased accumulation of proanthocyanidins (PAs) in the seed coat results in darker seeds in many market classes of colored beans after harvest. The precursors of PAs are synthesized in the cytoplasm, and subsequently get glycosylated and then transported to the vacuoles where polymerization occurs. Thus, vacuolar transporters play an important role in the accumulation of PAs. Here, we report that common bean genome contains 59 multidrug and toxic compound extrusion genes (PvMATEs). Phylogenetic analysis of putative PvMATEs with functionally characterized MATEs from other plant species categorized them into substrate-specific clades. Our data demonstrate that a vacuolar transporter PvMATE8 is expressed at a higher level in the pinto bean cultivar CDC Pintium (regular darkening) compared to 1533-15 (slow darkening). PvMATE8 localizes in the vacuolar membrane and rescues the PA deficient (tt12) mutant phenotype in Arabidopsis thaliana. Analysis of PA monomers in transgenic seeds together with wild-type and mutants suggests a possible feedback regulation of PA biosynthesis and accumulation. Identification of PvMATE8 will help better understand the mechanism of PA accumulation in common bean.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1664-462X
Relation: https://www.frontiersin.org/articles/10.3389/fpls.2022.1046597/full; https://doaj.org/toc/1664-462X
DOI: 10.3389/fpls.2022.1046597
URL الوصول: https://doaj.org/article/b6255764ec4b4062b9c395093595458a
رقم الأكسشن: edsdoj.b6255764ec4b4062b9c395093595458a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1664462X
DOI:10.3389/fpls.2022.1046597