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Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis

التفاصيل البيبلوغرافية
العنوان: Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis
المؤلفون: Pan Huang, Fan Hu, Zhi‐Bo Yang, Yi Pan, Rong Zhou, Yi‐Ning Yan, Hai‐Zhen Wang, Chang Wang
المصدر: Kaohsiung Journal of Medical Sciences, Vol 39, Iss 5, Pp 501-510 (2023)
بيانات النشر: Wiley, 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine (General)
مصطلحات موضوعية: atopic dermatitis, Hsp90, keratinocytes, matrine, Th1/Th2 inflammatory response, Medicine (General), R5-920
الوصف: Abstract Atopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine Sophora flavescens, and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and its underlying mechanism. An AD mouse model and inflamed human epidermal keratinocyte cells (HaCaT) cells were established. Histopathological aspects were examined using hematoxylin and eosin staining, toluidine blue staining, and immunohistochemistry. The mRNA and protein expressions were assessed using quantitative real‐time polymerase chain reaction and Western blot, respectively. The secretions of cytokines and chemokines were examined by enzyme‐linked immunosorbent assay. Flow cytometry was carried out to analyze the proportions of T‐helper (Th) 1 and Th2 cells. Herein, our results displayed that matrine diminished AD symptoms and decreased heat shock protein 90 (Hsp90) expression. Matrine decreased the Th2 cytokine levels in the ear tissues and serum, and it also significantly repressed inflammatory cytokines (thymus activation regulated chemokine and interleukin‐6) secretions by repressing the Hsp90/NF‐κB signaling axis in inflamed HaCaT cells. Furthermore, matrine inhibited Th2 differentiation of CD4+ T cells when co‐cultured with inflamed HaCaT cells. Matrine can regulate the Th1/Th2 inflammatory response by inhibiting the Hsp90/NF‐κB signaling axis to alleviate AD. Therefore, it may be a candidate for AD treatment.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2410-8650
1607-551X
Relation: https://doaj.org/toc/1607-551X; https://doaj.org/toc/2410-8650
DOI: 10.1002/kjm2.12655
URL الوصول: https://doaj.org/article/b67612e165084f3b917393d54e98fd8e
رقم الأكسشن: edsdoj.b67612e165084f3b917393d54e98fd8e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:24108650
1607551X
DOI:10.1002/kjm2.12655