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Mild Oxidative Damage in the Diabetic Rat Heart Is Attenuated by Glyoxalase-1 Overexpression

التفاصيل البيبلوغرافية
العنوان: Mild Oxidative Damage in the Diabetic Rat Heart Is Attenuated by Glyoxalase-1 Overexpression
المؤلفون: Casper G. Schalkwijk, Coen D. A. Stehouwer, Geja Hageman, Ben J. A. Janssen, Frans van Nieuwenhoven, Toshio Miyata, Joyce M. J. de Vos-Houben, Petra M. G. Niessen, Olaf Brouwers
المصدر: International Journal of Molecular Sciences, Vol 14, Iss 8, Pp 15724-15739 (2013)
بيانات النشر: MDPI AG, 2013.
سنة النشر: 2013
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: glycation, oxo-aldehydes, glyoxalase-I, oxidative stress, cardiac function, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Diabetes significantly increases the risk of heart failure. The increase in advanced glycation endproducts (AGEs) and oxidative stress have been associated with diabetic cardiomyopathy. We recently demonstrated that there is a direct link between AGEs and oxidative stress. Therefore, the aim of the current study was to investigate if a reduction of AGEs by overexpression of the glycation precursor detoxifying enzyme glyoxalase-I (GLO-I) can prevent diabetes-induced oxidative damage, inflammation and fibrosis in the heart. Diabetes was induced in wild-type and GLO-I transgenic rats by streptozotocin. After 24-weeks of diabetes, cardiac function was monitored with ultrasound under isoflurane anesthesia. Blood was drawn and heart tissue was collected for further analysis. Analysis with UPLC-MSMS showed that the AGE Nε-(1-carboxymethyl)lysine and its precursor 3-deoxyglucosone were significantly elevated in the diabetic hearts. Markers of oxidative damage, inflammation, and fibrosis were mildly up-regulated in the heart of the diabetic rats and were attenuated by GLO-I overexpression. In this model of diabetes, these processes were not accompanied by significant changes in systolic heart function, i.e., stroke volume, fractional shortening and ejection fraction. This study shows that 24-weeks of diabetes in rats induce early signs of mild cardiac alterations as indicated by an increase of oxidative stress, inflammation and fibrosis which are mediated, at least partially, by glycation.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/14/8/15724; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms140815724
URL الوصول: https://doaj.org/article/ceb6ef4724a847b88ccf4d6b59408122
رقم الأكسشن: edsdoj.b6ef4724a847b88ccf4d6b59408122
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms140815724