دورية أكاديمية
MicroRNAs and Their Associated Genes Regulating the Acrosome Reaction in Sperm of High- versus Low-Fertility Holstein Bulls
| العنوان: | MicroRNAs and Their Associated Genes Regulating the Acrosome Reaction in Sperm of High- versus Low-Fertility Holstein Bulls |
|---|---|
| المؤلفون: | Vanmathy Kasimanickam, Ramanathan Kasimanickam |
| المصدر: | Animals, Vol 14, Iss 6, p 833 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Veterinary medicine LCC:Zoology |
| مصطلحات موضوعية: | dairy bulls, fertility, spermatozoa, acrosome reaction, microRNA, mRNA, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| الوصف: | Bioinformatics envisage experimental data as illustrated biological networks, exploring roles of individual proteins and their interactions with other proteins in regulation of biological functions. The objective was to identify differentially expressed miRNAs and their associated genes regulating the acrosome reaction in capacitated sperm of high- compared to low-fertility dairy bulls and to elucidate biological functional pathways using a systems biology approach, featuring miRNA–mRNA cluster analysis. Categorized bovine-specific miRNAs (n = 84) were analyzed by RT-PCR; 19 were differentially expressed in high- compared to low-fertility sperm (p ≤ 0.05, fold regulation ≥ 2 magnitudes). Six miRNAs (bta-miR-129-5p, bta-miR-193a-3p, bta-miR-217, bta-mir-296-5p, bta-miR-27a, and bta-miR-320a) were highly upregulated (p < 0.05; fold regulation ≥ 5 magnitudes) in high- compared to low-fertility sperm. Highly scored integrated genes of differentially expressed miRNAs predicted associations with pathways regulating acrosome vesicle exocytosis, acrosome reaction, and sperm-oocyte binding. The mRNA expressions of genes associated with the acrosome reaction (including hub genes) were greater, ranging from 2.0 to 9.1-fold (p < 0.05) in high- compared to low-fertility capacitated bull sperm. In conclusion, differentially expressed miRNAs in high-fertility bovine sperm regulating acrosome functions have potential for predicting bull fertility. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 14060833 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/14/6/833; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani14060833 |
| URL الوصول: | https://doaj.org/article/cb6fa06e6d9940289aa3e7df5c5f17bf |
| رقم الأكسشن: | edsdoj.b6fa06e6d9940289aa3e7df5c5f17bf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14060833 20762615 |
|---|---|
| DOI: | 10.3390/ani14060833 |