دورية أكاديمية
Novel ATP-cone-driven allosteric regulation of ribonucleotide reductase via the radical-generating subunit
| العنوان: | Novel ATP-cone-driven allosteric regulation of ribonucleotide reductase via the radical-generating subunit |
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| المؤلفون: | Inna Rozman Grinberg, Daniel Lundin, Mahmudul Hasan, Mikael Crona, Venkateswara Rao Jonna, Christoph Loderer, Margareta Sahlin, Natalia Markova, Ilya Borovok, Gustav Berggren, Anders Hofer, Derek T Logan, Britt-Marie Sjöberg |
| المصدر: | eLife, Vol 7 (2018) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | Leewenhoekiella blandensis, ribonucleotide reductase, allosteric regulation, dATP inhibition, ATP-cone, oligomerization, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Ribonucleotide reductases (RNRs) are key enzymes in DNA metabolism, with allosteric mechanisms controlling substrate specificity and overall activity. In RNRs, the activity master-switch, the ATP-cone, has been found exclusively in the catalytic subunit. In two class I RNR subclasses whose catalytic subunit lacks the ATP-cone, we discovered ATP-cones in the radical-generating subunit. The ATP-cone in the Leeuwenhoekiella blandensis radical-generating subunit regulates activity via quaternary structure induced by binding of nucleotides. ATP induces enzymatically competent dimers, whereas dATP induces non-productive tetramers, resulting in different holoenzymes. The tetramer forms by interactions between ATP-cones, shown by a 2.45 Å crystal structure. We also present evidence for an MnIIIMnIV metal center. In summary, lack of an ATP-cone domain in the catalytic subunit was compensated by transfer of the domain to the radical-generating subunit. To our knowledge, this represents the first observation of transfer of an allosteric domain between components of the same enzyme complex. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/31529; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.31529 |
| URL الوصول: | https://doaj.org/article/b748293d383c40c1839ef9c37f256aa8 |
| رقم الأكسشن: | edsdoj.b748293d383c40c1839ef9c37f256aa8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.31529 |