دورية أكاديمية
Simultaneous Structural Identification of Natural Products in Fractions of Crude Extract of the Rare Endangered Plant Anoectochilus roxburghii Using 1H NMR/RRLC-MS Parallel Dynamic Spectroscopy
| العنوان: | Simultaneous Structural Identification of Natural Products in Fractions of Crude Extract of the Rare Endangered Plant Anoectochilus roxburghii Using 1H NMR/RRLC-MS Parallel Dynamic Spectroscopy |
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| المؤلفون: | Zeper Abliz, Rui-Ping Zhang, Wen-Yi He, Shun-Xing Guo, Xiao-Xue Wang, Chun-Lan Wang, Jiu-Ming He, Rui-Xiang Sun |
| المصدر: | International Journal of Molecular Sciences, Vol 12, Iss 4, Pp 2556-2571 (2011) |
| بيانات النشر: | MDPI AG, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | 1H NMR/RRLC-MS parallel dynamic spectroscopy, flavonoids, organic acids, crude extract, Anoectochilus roburghii, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Nuclear magnetic resonance/liquid chromatography-mass spectroscopy parallel dynamic spectroscopy (NMR/LC-MS PDS) is a method aimed at the simultaneous structural identification of natural products in complex mixtures. In this study, the method is illustrated with respect to 1H NMR and rapid resolution liquid chromatography-mass spectroscopy (RRLC-MS) data, acquired from the crude extract of Anoectochilus roxburghii, which was separated into a series of fractions with the concentration of constituent dynamic variation using reversed-phase preparative chromatography. Through fraction ranges and intensity changing profiles in 1H NMR/RRLC–MS PDS spectrum, 1H NMR and the extracted ion chromatogram (XIC) signals deriving from the same individual constituent, were correlated due to the signal amplitude co-variation resulting from the concentration variation of constituents in a series of incompletely separated fractions. 1H NMR/RRLC-MS PDS was then successfully used to identify three types of natural products, including eight flavonoids, four organic acids and p-hydroxybenzaldehyde, five of which have not previously been reported in Anoectochilus roxburghii. In addition, two groups of co-eluted compounds were successfully identified. The results prove that this approach should be of benefit in the unequivocal structural determination of a variety of classes of compounds from extremely complex mixtures, such as herbs and biological samples, which will lead to improved efficiency in the identification of new potential lead compounds. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | http://www.mdpi.com/1422-0067/12/4/2556/; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms12042556 |
| URL الوصول: | https://doaj.org/article/b769e401df8d4f9e90b7c313677df9b2 |
| رقم الأكسشن: | edsdoj.b769e401df8d4f9e90b7c313677df9b2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms12042556 |