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Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn

التفاصيل البيبلوغرافية
العنوان: Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn
المؤلفون: Fang Bian, Yangyan Xiao, Mahira Zaheer, Eugene A. Volpe, Stephen C. Pflugfelder, De-Quan Li, Cintia S. de Paiva
المصدر: International Journal of Molecular Sciences, Vol 18, Iss 3, p 562 (2017)
بيانات النشر: MDPI AG, 2017.
سنة النشر: 2017
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: alkali injury, NLRP3 inflammasome, sodium butyrate, β-hydroxybutyric acid, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Epithelial cells are involved in the regulation of innate and adaptive immunity in response to different stresses. The purpose of this study was to investigate if alkali-injured corneal epithelia activate innate immunity through the nucleotide-binding oligomerization domain-containing protein (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome pathway. A unilateral alkali burn (AB) was created in the central cornea of C57BL/6 mice. Mice received either no topical treatment or topical treatment with sodium butyrate (NaB), β-hydroxybutyric acid (HBA), dexamethasone (Dex), or vehicle (balanced salt solution, BSS) quater in die (QID) for two or five days (d). We evaluated the expression of inflammasome components including NLRP3, apoptosis-associated speck-like protein (ASC), and caspase-1, as well as the downstream cytokine interleukin (IL)-1β. We found elevation of NLRP3 and IL-1β messenger RNA (mRNA) transcripts, as well as levels of inflammasome component proteins in the alkali-injured corneas compared to naïve corneas. Treatment with NLRP3 inhibitors using NaB and HBA preserved corneal clarity and decreased NLRP3, caspase-1, and IL-1β mRNA transcripts, as well as NLRP3 protein expression on post-injury compared to BSS-treated corneas. These findings identified a novel innate immune signaling pathway activated by AB. Blocking the NLRP3 pathway in AB mouse model decreases inflammation, resulting in greater corneal clarity. These results provide a mechanistic basis for optimizing therapeutic intervention in alkali injured eyes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/18/3/562; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms18030562
URL الوصول: https://doaj.org/article/b8242dadf55644b88904aacadbc8116e
رقم الأكسشن: edsdoj.b8242dadf55644b88904aacadbc8116e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms18030562