دورية أكاديمية
Probing cellular behaviors through nanopatterned chitosan membranes
| العنوان: | Probing cellular behaviors through nanopatterned chitosan membranes |
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| المؤلفون: | Chung-Yao Yang, Chun-Yen Sung, Hung-Hsun Shuai, Chao-Min Cheng and J Andrew Yeh |
| المصدر: | Science and Technology of Advanced Materials, Vol 14, Iss 4, p 044406 (2013) |
| بيانات النشر: | Taylor & Francis Group, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biotechnology |
| مصطلحات موضوعية: | Materials of engineering and construction. Mechanics of materials, TA401-492, Biotechnology, TP248.13-248.65 |
| الوصف: | This paper describes a high-throughput method for developing physically modified chitosan membranes to probe the cellular behavior of MDCK epithelial cells and HIG-82 fibroblasts adhered onto these modified membranes. To prepare chitosan membranes with micro/nanoscaled features, we have demonstrated an easy-to-handle, facile approach that could be easily integrated with IC-based manufacturing processes with mass production potential. These physically modified chitosan membranes were observed by scanning electron microscopy to gain a better understanding of chitosan membrane surface morphology. After MDCK cells and HIG-82 fibroblasts were cultured on these modified chitosan membranes for various culture durations (i.e. 1, 2, 4, 12 and 24 h), they were investigated to decipher cellular behavior. We found that both cells preferred to adhere onto a flat surface rather than on a nanopatterned surface. However, most (> 80%) of the MDCK cells showed rounded morphology and would suspend in the cultured medium instead of adhering onto the planar surface of negatively nanopatterned chitosan membranes. This means different cell types (e.g. fibroblasts versus epithelia) showed distinct capabilities/preferences of adherence for materials of varying surface roughness. We also showed that chitosan membranes could be re-used at least nine times without significant contamination and would provide us consistency for probing cell–material interactions by permitting reuse of the same substrate. We believe these results would provide us better insight into cellular behavior, specifically, microscopic properties and characteristics of cells grown under unique, nanopatterned cell-interface conditions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1468-6996 1878-5514 |
| Relation: | https://doaj.org/toc/1468-6996; https://doaj.org/toc/1878-5514 |
| DOI: | 10.1088/1468-6996/14/4/044406 |
| URL الوصول: | https://doaj.org/article/b896901953634c3eac9b3d86f6dc18bf |
| رقم الأكسشن: | edsdoj.b896901953634c3eac9b3d86f6dc18bf |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 14686996 18785514 |
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| DOI: | 10.1088/1468-6996/14/4/044406 |