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Gene silencing of VP1 gene of coxsackievirus B3 neurotropic strain Nancy by dsRNAs and siRNAs

التفاصيل البيبلوغرافية
العنوان: Gene silencing of VP1 gene of coxsackievirus B3 neurotropic strain Nancy by dsRNAs and siRNAs
المؤلفون: Nikolay Petrov, Mariya Stoyanova, Adelina Stoyanova, Ivanka Nikolova, Petar Grozdanov, Angel Galabov
المصدر: Biotechnology & Biotechnological Equipment, Vol 36, Iss 1, Pp 353-361 (2022)
بيانات النشر: Taylor & Francis Group, 2022.
سنة النشر: 2022
المجموعة: LCC:Biotechnology
مصطلحات موضوعية: PTGS, VP1, Coxsackievirus B3, CVB3, dsRNAs, siRNAs, Biotechnology, TP248.13-248.65
الوصف: AbstractCoxsackieviruses are distributed worldwide and can cause serious diseases threatening human health and even human life. Genetic silencing is considered a new opportunity to treat viral infections. In this study, we produced in vitro dsRNAs and siRNAs specific for two sequences of different length from the VP1 genetic regions of CVB3, and introduced them into HEp-2 cells with Oligofectamine as the transfection agent. We used the neurotropic strain Nancy. The 100% inhibitory concentrations of the applied dsRNAs and siRNAs were 0.3 μmol/L and fell in the range of non-cytotoxic concentration values. The produced VP1-specific dsRNAs and siRNAs were effective at low concentrations, suggesting that the produced RNAs are safe to use and possess excellent potential to treat CVB3 infection.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 13102818
1314-3530
1310-2818
Relation: https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530
DOI: 10.1080/13102818.2022.2082320
URL الوصول: https://doaj.org/article/b8c62bf0a1a94ed0a825178ab31f8495
رقم الأكسشن: edsdoj.b8c62bf0a1a94ed0a825178ab31f8495
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:13102818
13143530
DOI:10.1080/13102818.2022.2082320