دورية أكاديمية
Baicalin attenuates LPS-induced alveolar type II epithelial cell A549 injury by attenuation of the FSTL1 signaling pathway via increasing miR-200b-3p expression
| العنوان: | Baicalin attenuates LPS-induced alveolar type II epithelial cell A549 injury by attenuation of the FSTL1 signaling pathway via increasing miR-200b-3p expression |
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| المؤلفون: | Xin-ya Duan, Yang Sun, Zhu-fen Zhao, Yao-qing Shi, Xun-yan Ma, Li Tao, Ming-wei Liu |
| المصدر: | Innate Immunity, Vol 27 (2021) |
| بيانات النشر: | SAGE Publishing, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607 |
| الوصف: | In China, baicalin is the main active component of Scutellaria baicalensis , which has been used in the treatment of inflammation-related diseases, such as inflammation-induced acute lung injury. However, its specific mechanism remains unclear. This study examined the protective effect of baicalin on LPS-induced inflammation injury of alveolar epithelial cell line A549 and explored its protective mechanism. Compared with the LPS-induced group, the proliferation inhibition rates of alveolar type II epithelial cell line A549 intervened by different concentrations of baicalin decreased significantly, as did the levels of inflammatory factors IL-6, IL-1β, prostaglandin 2 and TNF-α in the supernatant. The expression levels of inflammatory proteins inducible NO synthase (iNOS), NF-κB65, phosphorylated ERK (p-ERK1/2), and phosphorylated c-Jun N-terminal kinase (p-JNK1) significantly decreased, as did the protein expression of follistatin-like protein 1 (FSTL1). In contrast, expression of miR-200b-3p significantly increased in a dose-dependent manner. These results suggested that baicalin could significantly inhibit the expression of inflammation-related proteins and improve LPS-induced inflammatory injury in alveolar type II epithelial cells. The mechanism may be related to the inhibition of ERK/JNK inflammatory pathway activation by increasing the expression of miR-200b-3p. Thus, FSTL1 is the regulatory target of miR-200b-3p. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1753-4259 1753-4267 17534259 |
| Relation: | https://doaj.org/toc/1753-4259; https://doaj.org/toc/1753-4267 |
| DOI: | 10.1177/17534259211013887 |
| URL الوصول: | https://doaj.org/article/db9021625e4e4f6f8d1cd01395b7273e |
| رقم الأكسشن: | edsdoj.b9021625e4e4f6f8d1cd01395b7273e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17534259 17534267 |
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| DOI: | 10.1177/17534259211013887 |