دورية أكاديمية
Human amniotic epithelial cell-derived extracellular vesicles provide an extracellular matrix-based microenvironment for corneal injury repair
| العنوان: | Human amniotic epithelial cell-derived extracellular vesicles provide an extracellular matrix-based microenvironment for corneal injury repair |
|---|---|
| المؤلفون: | Shuqin Hu, Zhe Wang, Caixia Jin, Qizhen Chen, Yuchen Fang, Jiahui Jin, Jie Chen, Lixia Lu, Haibin Tian, Jingying Xu, Furong Gao, Juan Wang, Jieping Zhang, Hong-Ping Cui, Guo-Tong Xu, Qingjian Ou |
| المصدر: | Journal of Tissue Engineering, Vol 13 (2022) |
| بيانات النشر: | SAGE Publishing, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biochemistry |
| مصطلحات موضوعية: | Biochemistry, QD415-436 |
| الوصف: | To study the biological functions and applications of human amniotic epithelial cell-derived extracellular vesicles (hAEC-EVs), the cargos of hAEC-EVs were analyzed using miRNA sequencing and proteomics analysis. The hAECs and hAEC-EVs in this study had specific characteristics. Multi-omics analyses showed that extracellular matrix (ECM) reorganization, inhibition of excessive myofibroblasts, and promotion of target cell adhesion to the ECM were their primary functions. We evaluated the application of hAEC-EVs for corneal alkali burn healing in rabbits and elucidated the fundamental mechanisms. Slit-lamp images revealed that corneal alkali burns induced central epithelial loss, stromal haze, iris, and pupil obscurity in rabbits. Slit-lamp examination and histological findings indicated that hAEC-EVs facilitated re-epithelialization of the cornea after alkali burns, reduced scar formation and promoted the restoration of corneal tissue transparency. Significantly fewer α-SMA-positive myofibroblasts were observed in the hAEC-EV-treated group than the PBS group. HAEC-EVs effectively promoted the proliferation and migration of hCECs and hCSCs in vitro and activated the focal adhesion signaling pathway. We demonstrated that hAEC-EVs were excellent cell-free candidates for the treatment of ECM lesion-based diseases, including corneal alkali burns. HAEC-EVs promoted ECM reorganization and cell adhesion of target tissues or cells via orderly activation of the focal adhesion signaling pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2041-7314 20417314 |
| Relation: | https://doaj.org/toc/2041-7314 |
| DOI: | 10.1177/20417314221122123 |
| URL الوصول: | https://doaj.org/article/db911552d0ae43a19ad24a3ad032a24f |
| رقم الأكسشن: | edsdoj.b911552d0ae43a19ad24a3ad032a24f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20417314 |
|---|---|
| DOI: | 10.1177/20417314221122123 |