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Toxicarioside A inhibits tumor growth and angiogenesis: involvement of TGF-β/endoglin signaling.

التفاصيل البيبلوغرافية
العنوان: Toxicarioside A inhibits tumor growth and angiogenesis: involvement of TGF-β/endoglin signaling.
المؤلفون: Feng-Ying Huang, Wen-Li Mei, Yue-Nan Li, Guang-Hong Tan, Hao-Fu Dai, Jun-Li Guo, Hua Wang, Yong-Hao Huang, Huan-Ge Zhao, Song-Lin Zhou, Ying-Ying Lin
المصدر: PLoS ONE, Vol 7, Iss 11, p e50351 (2012)
بيانات النشر: Public Library of Science (PLoS), 2012.
سنة النشر: 2012
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Toxicarioside A is a cardenolide isolated mainly from plants and animals. Emerging evidence demonstrate that cardenolides not only have cardiac effects but also anticancer effects. In this study, we used in vivo models to investigate the antitumor activities of toxicarioside A and the potential mechanisms behind them. Murine colorectal carcinoma (CT26) and Lewis lung carcinoma (LL/2) models were established in syngeneic BALB/c and C57BL/6 mice, respectively. We found that the optimum effective dose of toxicarioside A treatment significantly suppressed tumor growth and angiogenesis in CT and LL/2 tumor models in vivo. Northern and Western blot analysis showed significant inhibition of endoglin expression in toxicarioside A-treated human umbilical vein endothelial cells (HUVECs) in vitro and tumor tissues in vivo. Toxicarioside A treatment significantly inhibited cell proliferation, migration and invasion, but did not cause significant cell apoptosis and affected other membrane protein (such as CD31 and MHC I) expression. In addition, TGF-β expression was also significantly inhibited in CT26 and LL/2 tumor cells treated with toxicarioside A. Western blot analysis indicated that Smad1 and phosphorylated Smad1 but not Smad2/3 and phosphorylated Smad2/3 were attenuated in HUVECs treated with toxicarioside A. Smad1 and Smad2/3 signaling remained unchanged in CT26 and LL/2 tumor cells treated with toxicarioside A. Endoglin knockout by small interfering RNA against endoglin induced alternations in Smad1 and Smad2/3 signaling in HUVECs. Our results indicate that toxicarioside A suppresses tumor growth through inhibition of endoglin-related tumor angiogenesis, which involves in the endoglin/TGF-β signal pathway.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3508932?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0050351
URL الوصول: https://doaj.org/article/b926181cd6434a9ea0919349abadd3db
رقم الأكسشن: edsdoj.b926181cd6434a9ea0919349abadd3db
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0050351