دورية أكاديمية
Spatial gene expression maps of the intestinal lymphoid follicle and associated epithelium identify zonated expression programs.
| العنوان: | Spatial gene expression maps of the intestinal lymphoid follicle and associated epithelium identify zonated expression programs. |
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| المؤلفون: | Noam Cohen, Hassan Massalha, Shani Ben-Moshe, Adi Egozi, Milena Rozenberg, Keren Bahar Halpern, Shalev Itzkovitz |
| المصدر: | PLoS Biology, Vol 19, Iss 10, p e3001214 (2021) |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | The intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related to the scarcity and small sizes of ILFs and their follicle-associated epithelium (FAE) impeded the characterization of their spatial gene expression programs. Here, we combined RNA sequencing of laser capture microdissected tissues with single-molecule transcript imaging to obtain a spatial gene expression map of the ILF and its associated FAE in the mouse small intestine. We identified zonated expression programs in both follicles and FAEs, with a decrease in enterocyte antimicrobial and absorption programs and a partial induction of expression programs normally observed at the villus tip. We further identified Lepr+ subepithelial telocytes at the FAE top, which are distinct from villus tip Lgr5+ telocytes. Our analysis exposes the epithelial and mesenchymal cell states associated with ILFs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1544-9173 1545-7885 |
| Relation: | https://doaj.org/toc/1544-9173; https://doaj.org/toc/1545-7885 |
| DOI: | 10.1371/journal.pbio.3001214 |
| URL الوصول: | https://doaj.org/article/b9bbf4c2f6db41be9fc3a64ef0c9b256 |
| رقم الأكسشن: | edsdoj.b9bbf4c2f6db41be9fc3a64ef0c9b256 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15449173 15457885 |
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| DOI: | 10.1371/journal.pbio.3001214 |